The blood perfused isolated heart: characterization of the model
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- 作者：E. Pasini ; R. Solfrini ; T. Bachetti ; M. Marino ; P. Bernocchi ; F. Visioli and R. Ferrari
- 关键词：Key words Isolated heart – ; blood perfusion – ; myocardial metabolism – ; pentobarbital anaesthesia
- 刊名：Basic Research in Cardiology
- 出版年：1999
- 出版时间：June 1999
- 年：1999
- 卷：94
- 期：3
- 页码：215-222
- 全文大小：98 KB
文摘
We have characterized the aerobic blood-perfused isolated heart model evaluating the hemodynamics and metabolism of both the blood donor animal and the isolated organ. Anaesthesia of the blood donor with sodium pentobarbital (30 mg/kg) increases arterial concentration of non esterified fatty acids (NEFA) from 80 ¡À 6 to 452 ¡À 70 μM; p < 0.01. Injection of 1.000 U/kg heparin causes a second significant increase from 452 ¡À 70 to 1012 ¡À 104 μM; p < 0.01. Insertion of the perfusion circuit, without the isolated heart, causes a reduction in blood pressure of the blood donor and a significant increase in norepinephrine from 277 ¡À 44 to 634 ¡À 130 pg/ml; p < 0.05.