The role of the transformer gene in sex determination and reproduction in the tephritid fruit fly, Bactrocera dorsalis (Hendel)

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• 作者：Wei Peng ; Wenping Zheng ; Alfred M. Handler ; Hongyu Zhang
• 关键词：Bactrocera dorsalis (Hendel) ; Transformer gene ; Transformer ; 2 gene ; Bdtra RNAi ; Tephritid sex determination
• 刊名：Genetica
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：143
• 期：6
• 页码：717-727
• 全文大小：1,893 KB
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• 作者单位：Wei Peng (1)
  Wenping Zheng (1)
  Alfred M. Handler (2)
  Hongyu Zhang (1)
  
  1. State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control and Institute of Urban and Horticultural Entomology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People鈥檚 Republic of China
  2. USDA/ARS, Center for Medical, Agricultural and Veterinary Entomology, 1700 SW 23rd Drive, Gainesville, FL, 32608, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Life Sciences
  Animal Genetics and Genomics
  Plant Genetics and Genomics
  Human Genetics
  Microbial Genetics and Genomics
  
• 出版者：Springer Netherlands
• ISSN：1573-6857

文摘

Transformer (tra) is a switch gene in the somatic sex-determination hierarchy that regulates sexual dimorphism based on RNA splicing in many insects. In tephritids, a Y-linked male determining gene (M) controls sex in the sex-determination pathway. Here, homologues of Drosophila tra and transformer-2 (tra-2) genes were isolated and characterized in Bactrocera dorsalis (Hendel), one of the most destructive agricultural insect pests in many Asian countries. Two male-specific and one female-specific isoforms of B. dorsalis transformer (Bdtra) were identified. The presence of multiple TRA/TRA-2 binding sites in Bdtra suggests that the TRA/TRA-2 proteins are splicing regulators promoting and maintaining, epigenetically, female sex determination by a tra positive feedback loop in XX individuals during development. The expression patterns of female-specific Bdtra transcripts during early embryogenesis shows that a peak appears at 15 h after egg laying. Using dsRNA to knock-down Bdtra expression in the embryo and adult stages, we showed that sexual formation is determined early in the embryo stage and that parental RNAi does not lead to the production of all male progeny as in Tribolium castaneum. RNAi results from adult abdominal dsRNA injections show that Bdtra has a positive influence on female yolk protein gene (Bdyp1) expression and fecundity. Keywords Bactrocera dorsalis (Hendel) Transformer gene Transformer-2 gene Bdtra RNAi Tephritid sex determination