Isolation and characterization of Panax ginseng geranylgeranyl-diphosphate synthase genes responding to drought stress

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• 作者：Shadi Rahimi ; Yu-Jin Kim ; Balusamy Sri Renuka Devi…
• 关键词：Drought ; Gene characterization ; Geranylgeranyl diphosphate synthase ; Organ Expression ; Panax ginseng
• 刊名：European Journal of Plant Pathology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：142
• 期：4
• 页码：747-758
• 全文大小：3,115 KB
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• 作者单位：Shadi Rahimi (2)
  Yu-Jin Kim (1) (2)
  Balusamy Sri Renuka Devi (2)
  Altanzul Khorolragchaa (2)
  Johan Sukweenadhi (2)
  Deok-Chun Yang (1) (2)
  
  2. Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea
  1. Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Geranylgeranyl-diphosphate synthases (GGDPS) catalyze branch point enzymatic reactions producing isoprenoid-derived products which are necessary for plant growth and responses to a wide range of biotic and abiotic stresses. In our study, full length geranylgeranyl-diphosphate synthase 1 (PgGGDPS1) and 2 (PgGGDPS2) cDNA were isolated and characterized from the flower of Panax ginseng and 4-year old P. ginseng cv. Gumpoong. The cDNA had open reading frame of 1032 and 1116?bp with a deduced amino acid sequence of 343 and 371 residues for GGDPS1 and GGDPS2, respectively. The calculated molecular mass of GGDPS1 and GGDPS2 were approximately 37.66 and 40.21?kDa with a predicated isoelectric point of 5.32 and 6.23 and predicted localization of plastid. A GenBank Blast X search revealed that the deduced amino acid of PgGGDPS1 shared a high degree of homology with GGDPS from Panax notoginseng. The transcription pattern of GGDPS genes was different at various developmental stages. Both GGDPS genes were highly expressed in aerial parts of the plant, especially in rapidly growing tissues such as 4-year old flower and stem tissues. Transcript level of PgGGDPS1 was differentially induced in ginseng not only during Pseudomonas syringae pv tomato infection but also after exposure to abiotic stresses. Our results suggested that the induction of GGDPS genes specifically PgGGDPS1 by drought stress may affect chlorophyll levels, intracellular GA content and accumulation of carotenoids as the precursor for higher production of ABA and possibly stomatal closure as the barrier for water loss.