Adaptive alterations on gill Na+, K+-ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water

详细信息    查看全文

• 作者：Feng Zhao ; Beibei Wu ; Gang Yang ; Tao Zhang ; Ping Zhuang
• 关键词：Acipenser sinensis ; Na+ ; K+ ; ATPase ; Mitochondria ; rich cell ; Osmoregulation ; The Yangtze Estuary
• 刊名：Fish Physiology and Biochemistry
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：42
• 期：2
• 页码：749-756
• 全文大小：1,162 KB
• 参考文献：Allen PJ, Cech JJ (2007) Age/size effects on juvenile green sturgeon (Acipenser medirostris), oxygen consumption, growth, and osmoregulation in saline environments. Environ Biol Fish 79:211–229CrossRef
  Allen PJ, Cech JJ, Kültz D (2009) Mechanisms of seawater acclimation in a primitive, anadromous fish, the green sturgeon. J Comp Physiol B 179:903–920CrossRef PubMed PubMedCentral
  Altinok I, Galli SM, Chapman FA (1998) Ionic and osmotic regulation capabilities of juvenile Gulf of Mexico sturgeon, Acipenser oxyrinchus desotoi. Comp Biochem Physiol A 120:609–616CrossRef
  Carmona R, Garcia-Gallego M, Sanz A, Domenzain A, Ostos-Garrido MV (2004) Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructure modifications in seawater-acclimated specimens. J Fish Biol 64:553–566CrossRef
  Cataldi E, Ciccotti E, Di Marco P, Disanto O, Bronzi P, Cataudella S (1995) Acclimation trials of juvenile Italian sturgeon to different salinities: morpho-physiological descriptors. J Fish Biol 47:609–618CrossRef
  Evans DH (2008) Teleost fish osmoregulation: what we learned since August Krogh, Homer Smith, and Ancel Keys. Am J Physiol Regul Integr Comp Physiol 295:704–713CrossRef
  Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97–177CrossRef PubMed
  Foskett JK, Logsdon CD, Turner T, Machen TE, Bern HA (1981) Differentiation of the chloride extrusion mechanism during seawater adaptation of a teleost fish, the cichlid. J Exp Biol 93:209–224
  He X, Zhuang P, Zhang L, Xie C (2009) Osmoregulation in juvenile Chinese sturgeon (Acipenser sinensis Gray) during brackish water adaptation. Fish Physiol Biochem 35:223–230CrossRef PubMed
  Hiroi J, McCormick SD (2012) New insights into gill ionocyte and ion transporter function in euryhaline and diadromous fish. Respir Physiol Neurobiol 184:257–268CrossRef PubMed
  Hirose S, Kaneko T, Naito N, Takei Y (2003) Molecular biology of major components of chloride cells. Comp Biochem Physiol B 136:593–620CrossRef PubMed
  Hsu HH, Lin LY, Tseng YC, Horng JL, Hwang PP (2014) A new model for fish ion regulation: identification of ionocytes in freshwater-and seawater-acclimated medaka (Oryzias latipes). Cell Tissue Res 357:225–243CrossRef PubMed
  Hwang PP, Lee TH (2007) New insights into fish ion regulation and mitochondrion-rich cells. Comp Biochem Physiol A 148:479–497CrossRef
  Hwang PP, Lee TH, Lin LY (2011) Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms. Am J Physiol Regul Integr Comp Physiol 301:28–47CrossRef
  Javis PL, Ballantyne JS (2003) Metabolic response to salinity acclimation in juvenile shortnose sturgeon Acipenser brevirostrum. Aquaculture 219:891–909CrossRef
  Keys AB, Willmer EM (1932) Chloride-secreting cells in the gills of fishes, with special reference to the common eel. J Physiol 76:368–378CrossRef PubMed PubMedCentral
  Krayushkina LS, Semenova OG (2006) Osmotic and ion regulation in different species of Acipenserids (Acipenseriformes, Acipenseridae). J Ichthyol 46:113–124CrossRef
  Laurent P, Chevalier C, Wood CM (2006) Appearance of cuboidal cells in relation to salinity in gills of Fundulus heteroclitus, a species exhibiting branchial Na+ but not Cl− uptake in freshwater. Cell Tissue Res 325:481–492CrossRef PubMed
  LeBreton GTO, Beamish FWH (1998) The influence of salinity on ionic concentrations and osmolarity of blood serum in lake sturgeon, Acipenser fulvescens. Enviorn Biol Fish 52:477–482CrossRef
  McCormick SD (1995) Hormonal control of gill Na+, K+-ATPase and chloride cell function. In: Wood CM, Shuttleworth TJ (eds) Cellular and molecular approaches to fish ionic regulation. Academic Press, New York, pp 285–315
  McCormick SD, Shrimpton JM, Zydlewski JD (1997) Temperature effects on osmoregulatory physiology of juvenile anadromous fish. In: Wood CM, McDonald DG (eds) Society for experimental biology seminar series; global warming: implications for freshwater and marine fish. Cambridge University Press, New York, pp 279–301CrossRef
  McEnroe M, Cech JJ Jr (1985) Osmoregulation in juvenile and adult white sturgeon Acipenser transmontanus. Environ Biol Fishes 14:23–30CrossRef
  McEnroe M, Cech JJ Jr (1987) Osmoregulation in white sturgeon: life history aspects. Am Fish Soc Symp 1:191–196
  McKenzie DJ, Cataldi E, Di Marco P, Mandich A, Romano P, Ansferri S, Bronzi P, Cataudella S (1999) Some aspects of osmotic and ionic regulation in Adriatic sturgeon Acipenser naccarii. II: morpho-physiological adjustments to hyperosmotic environments. J Appl Ichthyol 15:61–66CrossRef
  Mizuno S, Ura K, Okubo T, Chida Y, Misaka N, Adachi S, Yamauchi K (2000) Ultrastructural changes in gill chloride cells during smoltification in wild and hatchery-reared masu salmon Oncorhynchus masou. Fish Sci 66:670–677CrossRef
  Natochin YV, Lukianenko VI, Kirsanov VJ, Lavrova EA, Metallov GF, Shakhmatova EI (1985) Features of osmotic and ionic regulations in Russian sturgeon (Acipenser guldenstadti Brandt). Comp Biochem Physiol A 80:297–302CrossRef PubMed
  Rodriguez A, Gallardo MA, Gisbert E, Santilari S, Ibarz A, Sanchez J, Castello-Orvay F (2002) Osmoregulation in juvenile Siberian sturgeon (Acipenser baerii). Fish Physiol Biochem 26:345–354CrossRef
  Russell JM (2000) Sodium–potassium–chloride cotransport. Physiol Rev 80:211–276PubMed
  Sardella BA, Kültz D (2009) Osmo- and ionregulatory response of green sturgeon (Acipenser medirostris) to salinity acclimation. J Comp Physiol B 179:383–390CrossRef PubMed
  Shelukhin GK, Metallov GF, Geraskin PP (1990) Effect of temperature and Salinity of Caspian Sea water on juvenile Russian sturgeon, Acipenser guldenstadti. J Ichthyol 30:75–85
  Uchida K, Kaneko T, Yamauchi K, Hirano T (1996) Morphometrical analysis of chloride cell activity in the gill filaments and lamellae and changes in Na+, K+-ATPase activity during seawater adaptation in chum salmon fry. J Exp Zool 276:193–200CrossRef
  Wei Q, Ke F, Zhang J, Zhuang P, Luo J, Zhou R, Yang W (1997) Biology, fisheries, and conservation of sturgeons and paddlefish in China. Environ Biol Fishes 48:241–255CrossRef
  Wilson JM, Laurent P (2002) Fish gill morphology: inside out. Can J Zool A 293:192–213
  Wright PA (2007) Ionic, osmotic, and nitrogenous waste regulation. In: McKenzie DJ, Farrell AP, Brauner CJ (eds) Primitive fishes. Fish physiology series, vol 26. Academic Press, San Diego, pp 283–318CrossRef
  Zhang T, Zhuang P, Liu J, Zhang L, Feng G, Hou J, Zhao F, Liu J (2009) Species composition and biodiversity of fish community in Chongming Dongtan of Yangtze River estuary. Chin J Ecol 28:2056–2062 (in Chinese)
  Zhao F, Zhang L, Zhuang P, He X (2009) Physiological changes of juvenile Chinese sturgeon during seaward migration in the Yangtze Estuary. In: Zhuang P, Liu J, Wang Y, Zhang T, Chen J (eds) The Yangtze Estuary nature reserve for Chinese sturgeon: scientific studies and management. China Ocean Press, Beijing, pp 235–244 (in Chinese)
  Zhao F, Qu L, Zhuang P, Zhang L, Liu J, Zhang T (2011) Salinity tolerance as well as osmotic and ionic regulation in juvenile Chinese sturgeon (Acipenser sinesis Gray, 1835) exposed to different salinities. J Appl Ichthyol 27:231–234CrossRef
  Zhao F, Zhuang P, Zhang T, Zhang L, Liu J, Hou J (2014) Non-lethal gill biopsy of juvenile hybrid sturgeon (Acipenser ruthenus ♀ × A. schrenckii ♂): validity and impact on growth and osmoregulation. J Appl Ichthyol 30:1243–1245CrossRef
  Zhao F, Zhuang P, Zhang T, Wang Y, Hou J, Liu J, Zhang L (2015) Isosmotic points and their ecological significance for juvenile Chinese sturgeon Acipenser sinensis. J Fish Biol 86:1416–1420CrossRef PubMed
  Zydlewski J, McMormick SD (2001) Developmental and environmental regulation of chloride cells in young American shad, Alosa sapidissima. J Exp Zool 290:73–87CrossRef PubMed
  
• 作者单位：Feng Zhao (1)
  Beibei Wu (1)
  Gang Yang (1)
  Tao Zhang (1)
  Ping Zhuang (1)
  
  1. Key Laboratory of Fisheries Ecology of the Yangtze Estuary, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

Understanding the physiological changes and osmoregulatory strategy is critical for anadromous species to adapt to large changes between freshwater and marine environments. In this study, juvenile Chinese sturgeon (Acipenser sinensis) were acclimated for 2 months to freshwater (FW, c. 0 ‰) and brackish water (BW, 15 ‰). Blood was assessed for changes in osmolality and ions. Gill tissue was assayed for Na+, K+-ATPase (NKA) activity and immunohistochemical analysis on mitochondria-rich cells (MRCs). Serum osmolality and ions concentrations (Na+, Cl− and K+) examined, except K+, increased significantly in those specimens adapted to BW. However, the variations were within the range of effective hyperosmotic adaptation. The specific activity of gill NKA of juveniles adapted to BW was significantly higher (c. 1.6 times) than that of fish adapted to FW. MRCs were mainly presented in the interlamellar region of the filament and at the base of the lamella in either FW- or BW-acclimated individuals. In BW, the number and size of MRCs on filaments greatly increased. However, there was no significant difference in the number and size of the MRCs at the lamella region. Results show that juvenile Chinese sturgeon keep osmotic homeostasis in hyperosmotic environments by increasing gill NKA activity and MRCs’ size and number, which is similar to other sturgeons and euryhaline teleosts.