Double-stranded RNA in the biological control of grain aphid (Sitobion avenae F.)

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• 作者：Dahai Wang (1) (2)
  Qi Liu (3)
  Xia Li (4)
  Yongwei Sun (1)
  Hui Wang (1)
  Lanqin Xia (1)
  
  1. Institute of Crop Sciences/The National Key Facility for Crop Gene Resource and Genetic Improvement ; Chinese Academy of Agricultural Sciences (CAAS) ; 12 Zhongguanchun South Street ; Beijing ; 10081 ; China
  2. Beijing Autolab Biotechnology Co. Ltd. ; Eastern Campus ; Beijing Jiaotong University ; Beijing ; 100081 ; China
  3. The Medical College of Wenzhou ; Wenzhou ; 325032 ; China
  4. Institute of Zoology ; Chinese Academy of Sciences (CAS) ; 1 Beichen West Road ; Chaoyang District ; Beijing ; 100101 ; China
  
• 关键词：Grain aphid (Sitobion avenae F.) ; Transcriptome ; RNA interference (RNAi) ; Wheat (Triticum aestivum L.) ; Aphid control
• 刊名：Functional & Integrative Genomics
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：15
• 期：2
• 页码：211-223
• 全文大小：4,365 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Genetics and Genomics
  Microbial Genetics and Genomics
  Biochemistry
  Bioinformatics
  Animal Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-7948

文摘

Grain aphid (Sitobion avenae F.) is the most dominant and destructive pest of wheat, which causes significant yield loss of cereal plants each year by inflicting damage both through the direct effects of feeding and by vectoring debilitating plant viruses. In this study, we performed de novo transcriptome sequencing of grain aphid via Roche 454 GS-FLX pyrosequencing. A total of 1,106,696 reads were obtained and assembled into 32,277 unigenes, of which 25,389, 21,635, and 16,211 unigenes matched the Nt, Nr, and Swiss-Prot databases, respectively. Functional annotation of these unigenes revealed not only the presence of genes that encode the key components of RNAi machinery such as Dicer and Argonaute but also the genes encoding the TAR RNA binding protein (TRBP) and the SID-1 protein, which function in assisting the RNA-induced silencing complex (RISC) formation in microRNA (miRNA) pathway and mediating a systemic RNA interference (RNAi) effect though a cellular uptake mechanism. Furthermore, among a set of 66 unigenes selected for a double-stranded RNA (dsRNA) artificial diet assay, four novel effective RNAi targets, which led to high mortality of aphids due to the down-regulation of the expression of the respective target gene, were identified. Moreover, the expansion of systemic RNAi effect in grain aphid was observed by adding the fluorescently labeled dsRNA in an artificial diet assay.