Whole-Genome Expression Analysis of Human Mesenchymal Stromal Cells Exposed to Ultrasmooth Tantalum vs. Titanium Oxide Surfaces
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- 作者：Claudia Stiehler (1) (2)
Cody Bünger (3) (4)
Rupert W. Overall (2)
Lo?c Royer (5)
Michael Schroeder (5)
Morten Foss (4)
Flemming Besenbacher (4) (6)
Mogens Kruh?ffer (7)
Moustapha Kassem (8) (9)
Klaus-Peter Günther (1) (2)
Maik Stiehler (1) (2) - 关键词：Cell culture ; Gene expression ; Mesenchymal stem cell ; Osteoblast ; Tantalum ; Titanium ; Biocompatibility ; Cell differentiation ; Pathway analysis ; Cell proliferation ; Oxidative stress ; NRF2 ; EGR1 ; IRF ; 1 ; IRF ; 8 ; NF ; Y ; p53
- 刊名：Cellular and Molecular Bioengineering
- 出版年：2013
- 出版时间：June 2013
- 年：2013
- 卷：6
- 期：2
- 页码：199-209
- 全文大小：710KB
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Cody Bünger (3) (4)
Rupert W. Overall (2)
Lo?c Royer (5)
Michael Schroeder (5)
Morten Foss (4)
Flemming Besenbacher (4) (6)
Mogens Kruh?ffer (7)
Moustapha Kassem (8) (9)
Klaus-Peter Günther (1) (2)
Maik Stiehler (1) (2)
1. Department of Orthopaedics and Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Technische Universit?t Dresden, Dresden, Germany
2. CRTD -Center for Regenerative Therapies Dresden, Technische Universit?t Dresden, Dresden, Germany
3. Orthopaedic Research Laboratory, Aarhus University Hospital, Aarhus, Denmark
4. Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark
5. BIOTEC, Technische Universit?t Dresden, Dresden, Germany
6. Department of Physics and Astronomy, Aarhus University, Aarhus, Denmark
7. AROS Applied Biotechnology A/S, Aarhus, Denmark
8. Department of Endocrinology, Odense University Hospital, Odense, Denmark
9. Stem Cell Unit, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia - ISSN：1865-5033
文摘
Durable osseointegration of metallic bone implants requires that progenitor cells attach, proliferate and differentiate on the implant surface. Previously, we demonstrated superior biocompatibility of human mesenchymal stromal cells (MSCs) cultivated on ultrasmooth tantalum (Ta) as compared to titanium (Ti) surface. The aim of this study was to extend the previous investigation of biocompatibility by monitoring temporal gene expression of MSCs on topographically comparable smooth Ta and Ti surfaces using whole-genome gene expression analysis. Total RNA samples from telomerase-immortalized human MSCs cultivated on plain sputter-coated surfaces of Ta or Ti for 1, 2, 4, and 8?days were hybridized to n?=?16 U133 Plus 2.0 arrays (Affymetrix?). Functional annotation, cluster and pathway analyses were performed. The vast majority of genes were differentially regulated after 4?days of cultivation and genes upregulated by MSCs exposed to Ta and Ti were predominantly related to the processes of differentiation and transcription, respectively. Functional annotation analysis of the 1,000 temporally most significantly regulated genes suggests earlier cellular differentiation on Ta compared to Ti surface. Key genes related to osteogenesis and cell adhesion were upregulated by MSCs exposed to Ta. We further identified differentially regulated candidate transcription factors, e.g., NRF2, EGR1, IRF-1, IRF-8, NF-Y, and p53 as well as relevant signaling pathways, e.g., p53 and mTOR, indicating e.g., differences in the Ta- and Ti-induced oxidative stress reactions at the cell/biomaterial interface. These findings suggest that Ta is a promising material for bone implants.