14CO2 labeling : a reliable technique for rapid measurement of total root exudation capacity and vascular sap flow in crop plants
详细信息 查看全文
- 作者：Bhupinder Singh (1)
Sumedha Ahuja (1)
Renu Pandey (2)
RK Singhal (3) - 关键词：Root exudation capacity ; Vascular sap flow ; Groundnut ; 14CO2 labeling
- 刊名：Journal of Radioanalytical and Nuclear Chemistry
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：302
- 期：3
- 页码：1315-1320
- 全文大小：356 KB
- 参考文献：1. Singh B, Ahuja S, Singhal RK, Venu Babu P (2013) Effect of gamma radiation on wheat plant growth due to impact on gas exchange characteristics and mineral nutrient uptake and utilization. J Radioanal Nucl Chem 298:249鈥?57. doi:10.1007/s10967-012-2342-5 CrossRef
2. Sumedha A, Kumar M, Kumar P, Gupta VK, Singhal RK, Yadav A, Singh B (2014) Metabolic and biochemical changes caused by gamma irradiation in plants. J Radioanal Nucl Chem 300:199鈥?12. doi:10.1007/s10967-014-2969-5 CrossRef
3. Grusak MA (2002) Plant macro- and micronutrient minerals encyclopedia of life sciences. John Wiley & Sons, Ltd, Hoboken, pp 1鈥?
4. Hoffland E (2006) Organic anion exudation by lowland rice (Oryza sativa L.) at zinc and phosphorus deficiency. Plant Soil 283:155鈥?62 CrossRef
5. Rico MI, Alvarez JM, Lopez-Valdivia LM, Novillo J, Obrador A (2009) Manganese and zinc in acidic agricultural soils from Central Spain, distribution and phytoavailability prediction with chemical extraction tests. Soil Sci 174:94鈥?04 CrossRef
6. Pati R, Mukhopadhyay D (2010) Forms of soil acidity and the distribution of DTPA-extractable micronutrients in some soils of West Bengal (India) Proceedings of the 19th World Congress of Soil Science, Soil Solutions for a Changing World 1鈥? August 2010, Brisbane, Australia. Published on DVD pp 14鈥?8
7. Lyon GH, James CRS, Graham RD (2008) Exploiting micronutrient interaction to optimize biofortification programs, The Case for Inclusion of Selenium and Iodine in the Harvest Plus Program. Nutr Rev 62:247鈥?52 CrossRef
8. Singh B, Seva Nayak D, Usha K (2010) Micronutrient deficiency: A global challenge and physiological approach to improve grain productivity under low zinc availability. In: Anjum NA (Ed) Plant nutrition and abiotic stress tolerance II. Plant stress 4 (Special Issue 2), 73鈥?6
9. Singh B, Pandey R (2003) Differences in root exudation among phosphorus-starved genotypes of maize and green gram and its relationship with phosphorus uptake. J Plant Nutr 26:2391鈥?401 CrossRef
10. Zaheer A, Gill MA, Qureshi RH (2001) Genotypic variations of phosphorus utilization efficiency of crops. J Plant Nutr 24:1149鈥?171 CrossRef
11. Chandan K (2012) Physiological and molecular characterization of phytosiderophore mediated phytoremediation of heavy metals. Doctorate Dissertation, PG School, IARI, New Delhi
12. Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London, p 889
13. Adegoke GO, Falade KO, Babalola OC (2004) Control of lipid oxidation and fungal spoilage of roasted peanut (Arachis hypogaea) using the spice Aframomum danielli. J Food Agri Environ 2:128鈥?31
14. Pandey R, Krishnapriya V, Kishora N, Singh SB, Singh B (2013) Shoot labelling with 14CO2: a technique for assessing total root carbon exudation under phosphorus stress. Indian J Plant Physiol 18(3):250鈥?62. doi:10.1007/s40502-013-0041-z CrossRef
15. Sumedha A, Singh B, Gupta VK, Singhal RK, Venu Babu P (2014) Very low dose gamma irradiation stimulates gaseous exchange and carboxylation efficiency, but inhibits vascular sap flow in Groundnut (Arachis hypogaea L.). Int J Radiation Biol 90:179鈥?86. doi:10.3109/09553002.2014.868615 CrossRef
16. Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. Lowa State University Press, Ames, p 480
17. Ahuja S, Malhotra PK, Bhatia VK, Prasad R (2008) Statistical package for agricultural research (SPAR 2.0). J Ind Soc Agric Stat 61:65鈥?4
18. Singh B, Datta PS (2010) Gamma irradiation to improve plant vigour, grain development, and yield attributes of wheat. Radiation Phys Chem 79:139鈥?43 CrossRef
19. Sakamoto W (2006) Protein degradation machineries in plastids. Ann Rev Plant Biol 57:599鈥?21 CrossRef
20. Bald谋k R, Aytekin H, Erer M (2011) Radioactivity measurements and radiation dose assessments due to natural radiation in Karab眉k (Turkey). J Radioanal Nucl Chem 289:297鈥?02 CrossRef
21. Singhal RK, Narayanan U, Rudran K (1998) Interception/deposition of airborne 85Sr, 131I, 137Cs by spinach, radish and beans plants in tropical rainfall. J Radioanal Nucl Chem 238(1鈥?):13鈥?0 CrossRef
22. Tufail M, Sabiha J, Akhtar N (2010) Assessment of annual effective dose from natural radioactivity intake through wheat grain produced in Faisalabad, Pakistan. J Radioanal Nucl Chem 283:585鈥?90 CrossRef
23. Singh B, Datta PS (2010) Effect of low dose gamma irradiation on plant and grain nutrition of wheat. Radiation Phys Chem 79:819鈥?25 CrossRef
24. Abo-hegazi AMT, Ragab AI, Moustafa AK (1988) Heritability and genetic variability for some characters of sunflower in M3 and M4 Generation after Irradiation. J Agric Res 13:3鈥?5
25. Singh B, Ahuja S, Singhal RK, Venu Babu P (2014) Radiosensitivity studies and radio stability of ribulose-1,5 bis-carboxylase and gas exchange characteristics in Wheat, Garden Pea, Field Pea, Spinach, and Okra. Water Air Soil Pollut. doi:10.1007/s11270-013-1815-7
26. Zhang FS, Ma J, Cao YP (1997) Phosphorus deficiency enhances root exudation of low-molecular weight organic acids and utilization of sparingly soluble inorganic phosphates by radish (Raphanus satiuvs L.) and rape (Brassica napus L.) plants. Plant Soil 196:261鈥?64 CrossRef
27. Veneklaas EJ, Stevens J, Cawthray GR, Turner S, Grigg AM, Lambers H (2003) Chickpea and white lupin rhizosphere carboxylates vary with soil properties and enhance phosphorus uptake. Plant Soil 248:187鈥?97 CrossRef
28. Gahoonia TS, Ali R, Malhotra RS, Jahoor A, Rahman MM (2007) Variation in root morphological and physiological traits and nutrient uptake of chickpea genotypes. J Plant Nutr 30:829鈥?41 CrossRef
29. Sugita R, Natsuko IK, Atsushi HYO, Keitaro T, Tomoko MN (2013) Nondestructive real-time radioisotope imaging system for visualizing 14C-labeled chemicals supplied as CO2 in plants using / Arabidopsis thaliana. J Radioanal Nucl Chem 298(2):1411鈥?416 CrossRef
30. Iida K (2013) Quantitative evaluation of the biosynthetic pathways leading to 未-aminolevulinic acid from the Shemin precursor glycine via the C5 pathway in / Arthrobacter hyalinus by analysis of 13C-labeled coproporphyrinogen III biosynthesized from [2-13C]glycine, [1-13C]acetate, and [2-13C]acetate using13C NMR spectroscopy. J Radioanal Nucl Chem 295(3):1819鈥?827 CrossRef - 作者单位：Bhupinder Singh (1)
Sumedha Ahuja (1)
Renu Pandey (2)
RK Singhal (3)
1. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110012, India
2. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India
3. Analytical Spectroscopy Section, Analytical Chemistry Division, Mod Lab, BARC, Trombay, Mumbai, 400085, India - ISSN：1588-2780
文摘
Root release of organic compounds and rate of the vascular sap flow are important for understanding the nutrient and the source-sink dynamics in plants, however, their determination is procedurally cumbersome and time consuming. We report here a simple method involving 14C labeling for rapid and reliable measurement of root exudates and vascular sap flow rate in a variable groundnut population developed through seed gamma irradiation using a cobalt source (60Co). An experimental hypothesis that a higher 14C level in the vascular sap would indicate a higher root release of carbon by the roots into the rhizosphere was verified.