Hydrogen peroxide is involved in abscisic acid-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress

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• 作者：Xue-Ping Li ; Qing-Qing Xu ; Wei-Biao Liao ; Zhan-Jun Ma…
• 关键词：Adventitious root ; Abscisic acid (ABA) ; Chlorophyll fluorescence ; Drought stress ; Enzyme activity ; Hydrogen peroxide (H2O2)
• 刊名：Journal of Plant Biology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：59
• 期：5
• 页码：536-548
• 全文大小：625 KB
• 刊物主题：Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics & Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1867-0725
• 卷排序：59

文摘

Abscisic acid (ABA) and hydrogen peroxide (H₂O₂) are important regulatory factors involved in plant development under adversity stress. Here, the involvement of H₂O₂ in ABA-induced adventitious root formation in cucumber (Cucumis sativus L.) under drought stress was determined. The results indicated that exogenous ABA or H₂O₂ promoted adventitious rooting under drought stress, with a maximal biological response at 0.5 μM ABA or 800 μM H₂O₂. The promotive effects of ABA-induced adventitious rooting under drought stress were suppressed by CAT or DPI, suggesting that endogenous H₂O₂ might be involved in ABA-induced adventitious rooting. ABA increased relative water content (RWC), leaf chlorophyll content, chlorophyll fluorescence parameters (Fv/Fm, ΦPS II and qP), water soluble carbohydrate (WSC) and soluble protein content, and peroxidase (POD), polyphenol oxidase (PPO) and indoleacetate oxidase (IAAO) activities, while decreasing transpiration rate. However, the effects of ABA were inhibited by H₂O₂ scavenger CAT. Therefore, H₂O₂ may be involved in ABA-induced adventitious root development under drought stress by stimulating water and chlorophyll content, chlorophyll fluorescence, carbohydrate and nitrogen content, as well as some enzyme activities.