Determination of twelve herbicides in tobacco by a combination of solid–liquid–solid dispersive extraction using multi-walled carbon nanotubes, dispersive liquid-liquid micro-extraction, and detection by GC with triple quadrupole mass spectrometry
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- 作者：Qie Gen Liao (1)
Yao Min Zhou (1)
Lin Guang Luo (1)
Li Bing Wang (2)
Xiao Hu Feng (2) - 关键词：Herbicides ; Tobacco ; Multi ; walled carbon nanotubes ; Solid–liquid–solid dispersive extraction ; Dispersive liquid–liquid micro ; extraction ; Gas chromatography–triple quadrupole mass spectrometry
- 刊名：Microchimica Acta
- 出版年：2014
- 出版时间：January 2014
- 年：2014
- 卷：181
- 期：1-2
- 页码：163-169
- 全文大小：245 KB
- 作者单位：Qie Gen Liao (1)
Yao Min Zhou (1)
Lin Guang Luo (1)
Li Bing Wang (2)
Xiao Hu Feng (2)
1. Key Laboratory for Quality and Safety of Agricultural Products, Agricultural Product Quality Safety and Standards Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, People’s Republic of China
2. Fuzhou Tobacco Company, Jiangxi Province, Fuzhou, 344000, People’s Republic of China - ISSN：1436-5073
文摘
We report on a method for the determination of twelve herbicides using solid–liquid–solid dispersive extraction (SLSDE), followed by dispersive liquid-liquid micro-extraction (DLLME) and quantitation by gas chromatography with triple quadrupole mass spectrometric detection. SLSDE was applied to the extraction of herbicides from tobacco samples using multi-walled carbon nanotubes (MWCNTs) as clean-up adsorbents. The effect of the quantity of MWCNTs on SLSDE, and of type and volume of extraction and disperser solvents and of salt effect on DLLME were optimized. Good linearity is obtained in the 5.0 - 500?μg?kg? concentration range, with regression coefficients of >0.99. Intra-day and inter-day repeatability, expressed as relative standard deviations, are between 3 and 9?%. The recoveries in case of herbicide-spiked tobacco at concentration levels of 20.0, 50.0 and 100.0?g?kg? ranged from 79 to 105?%, and LODs are between 1.5 and 6.1?μg?kg?. All the tobacco samples were found to contain butralin and pendimethalin at levels ranging from 15.8 to 500.0?μg?kg?. Figure Schematic diagram of herbicide extraction from tobacco samples by SLSDE-DLLME procedures. (a) sample solution containing herbicide and 10?mL acetonitril, (b) MWCNTs cleanup, (c) extract mixed with water, (d) addition of 100?μL of extraction solvent(chloroform) into mixed solution, (e) vortex mixer for 1?min, (f) phase separation after centrifugation. ?A method for analysis of 12 herbicides in tobacco samples was developed. ?MCNTs were used as sorbent, DLLME was further applied to purification and enrichment.. ?Butralin and pendimethalin were found in all tobacco samples.