Assembly of cellulases with synthetic protein scaffolds in vitro

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• 作者：Ting Yu ; Xin Gao ; Yuhong Ren ; Dongzhi Wei
• 关键词：Multienzyme ; Assembly ; Scaffold ; Cellulose
• 刊名：Bioresources and Bioprocessing
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：2
• 期：1
• 全文大小：790KB
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• 作者单位：Ting Yu (1)
  Xin Gao (1)
  Yuhong Ren (1)
  Dongzhi Wei (1)
  
  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai, 200237, People鈥檚 Republic of China
  
• 刊物类别：Biochemical Engineering; Environmental Engineering/Biotechnology; Industrial and Production Engineer
• 刊物主题：Biochemical Engineering; Environmental Engineering/Biotechnology; Industrial and Production Engineering;
• 出版者：Springer Berlin Heidelberg
• ISSN：2197-4365

文摘

Background Enzymatic cascades in metabolic pathways are spatially organized in such a way as to facilitate the flow of substrates. The construction of artificial cellulase complexes that mimic natural multienzyme assemblies can potentially enhance the capacity for cellulose hydrolysis. In this study, an artificial cellulase complex was constructed by tethering three cellulases to a synthetic protein scaffold.