Mapping of the RPc-1 locus for Phytophthora cactorum resistance in Fragaria vesca

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• 作者：Jahn Davik ; Håvard Eikemo ; May Bente Brurberg ; Daniel James Sargent
• 关键词：Fragaria vesca ; Phytophthora cactorum ; QTL ; Candidate gene ; Disease resistance
• 刊名：Molecular Breeding
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：35
• 期：11
• 全文大小：1,091 KB
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• 作者单位：Jahn Davik (1)
  Håvard Eikemo (1)
  May Bente Brurberg (1)
  Daniel James Sargent (2) (3)
  
  1. Norwegian Institute of Bioeconomy Research, Høgskoleveien 7, 1430, Aas, Norway
  2. Centre for Research and Innovation, Fondazione Edmund Mach, via Mach 1, 38010, San Michele all’Adige, TN, Italy
  3. Driscoll’s Genetics Limited, East Malling Enterprise Centre, East Malling, Kent, ME19 6BJ, UK
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

The woodland strawberry (Fragaria vesca) has become the model plant for the economically important, but genetically complex, octoploid F. × ananassa. Crown rot caused by the oomycete Phytophthora cactorum is a major problem for the strawberry industry and the identification and incorporation of efficient resistance genes into superior cultivars are important for breeding. In the present study, two experimental populations were used in inoculation experiments under controlled greenhouse condition. Studies of a sparse diallel cross between resistant and susceptible F. vesca genotypes concluded that resistance to crown rot is inherited as a dominant trait under nuclear control. Subsequently, an F₂ population derived from the grandparents Bukammen (resistant) and Haugastøl 3 (susceptible) collected in Norway, were phenotyped in infection experiments and genotyped using genotyping-by-sequencing. A 416.2-cM linkage map was constructed, and a single major gene locus was identified on linkage group 6 that we attributed to resistance to Phytopthora infection. We propose to name the resistance locus RPc-1 (Resistance to Phytophthora cactorum 1). Gene prediction of the 3.3 Mb QTL recovered 801 genes of which 69 had a potential role in plant disease resistance.