Degradation of Plasticizer Di-n-butylphthalate by Delftia sp. TBKNP-05

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• 作者：Neelakanteshwar K. Patil ; Rajesh Kundapur ; Yogesh S. Shouche and T. B. Karegoudar
• 刊名：Current Microbiology
• 出版年：2006
• 出版时间：May 2006
• 年：2006
• 卷：52
• 期：5
• 页码：369-374
• 全文大小：319 KB

文摘

Bacterial strain Delftia sp. TBKNP-05, isolated by para-hydroxybenzoate enrichment technique, is capable of degrading di-n-butylphthalate (DBP) as a sole source of carbon and energy. Analysis of intermediates by thin-layer chromatography and high-performance liquid chromatography indicated the presence of monobutylphthalate (MBP), phthalate (PA), and protocatechuate (PCA). The washed cells grown on DBP and PA showed appreciable oxidation of DBP, MBP, PA, and PCA. The enzyme activities in cell-free extracts of Delftia sp. TBKNP-05 exhibited the presence of DBP esterase, MBP esterase, PA-dioxygenase, and PCA 4,5-dioxygenase. The PCA is metabolized by meta-cleavage pathway, leading to further mineralization of the compound in this bacterium.