Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract
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- 作者：Massoume Amini ; Ali Deljou…
- 关键词：Somatic embryo ; Cuscuta campestris extract ; Medicago sativa L. ; Embryo maturation
- 刊名：Physiology and Molecular Biology of Plants
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：22
- 期：3
- 页码：321-330
- 全文大小：1,498 KB
- 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
- 出版者：Springer India
- ISSN：0974-0430
- 卷排序：22
文摘
Developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. One of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. For investigating the influence of Cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to explants using Agrobacterium tumefaciens, and transgenic plants were recovered using medium supplemented with different concentration of the extract. Transgenic plants were analyzed by PCR and RT-PCR. Somatic embryos of Medicago sativa L. developed into plantlets at high frequency level (52 %) in the maturation medium supplemented with 50 mg 1−1C. campestris extract as compared to the medium without extract (26 %). Transformation efficiency was 29.3 and 15.2 % for medium supplemented with dodder extract and without the extract, respectively. HPLC and GC/MS analysis of the extract indicated high level of ABA and some compounds such as Phytol, which can affect the somatic embryo maturation. The antibacterial assay showed that the extract was effective against some strains of A. tumefaciens. These results have provided a scientific basis for using of C. campestris extract as a good natural source of antimicrobial agents and plant growth regulator as well, that can be used in tissue culture of transgenic plants.