Differentially regulated functional gene clusters identified in early hypoxic cardiomyocytes

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• 作者：Do Kyun Kim (1)
  Eunmi Choi (23)
  Byeong-Wook Song (34)
  Min-Ji Cha (34)
  Onju Ham (34)
  Se-Yeon Lee (34)
  Chang Youn Lee (5)
  Jun-Hee Park (5)
  Heesang Song (6)
  Ki-Chul Hwang (2347) kchwang@yuhs.ac
• 关键词：Cardiomyocytes &#8211 ; Microarray &#8211 ; Early ischemia &#8211 ; PKC &#8211 ; Calcium
• 刊名：Molecular Biology Reports
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：39
• 期：10
• 页码：9549-9556
• 全文大小：591.9 KB
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• 作者单位：1. Division of Thoracic and Cardiovascular Surgery, National Health Insurance Cooperated Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeongi-do, Republic of Korea2. Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, 120-752 Republic of Korea3. Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul, 120-752 Republic of Korea4. Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752 Republic of Korea5. Department of Integrated Omoics for Biomedical Sciences, Graduate School, Yonsei University, Seoul, 120-749 Republic of Korea6. Department of Biochemistry and Molecular Biology, Chosun University School of Medicine, Gwangju, 501-759 Republic of Korea7. Severance Biomedical Science Institute, Cardiovascular Research Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752 Republic of Korea
• ISSN：1573-4978

文摘

Pathological stress including myocardial infarction and hypertension causes a negative effect on calcium regulation and homeostasis. Nevertheless, few studies reveal that Ca2+ regulatory genes are related to pathological status in cardiomyocytes under early hypoxia. To determine the alteration of Ca2+-related gene in hypoxic myocytes, primary neonatal rat ventricular cardiomyocytes (NRVCMs) was isolated. Survival of hypoxic NRVCMs was significantly decreased in 6 h. We confirmed an increase of reactive oxygen species (ROS) generation and Ca2+ overload in hypoxic NRVCMs by using 2′,7′-dichlorodihydro-fluorescein diacetate (H2DCFDA) and FACS analysis. Furthermore, survival/apoptotic signals were also regulated in same condition. The expression profiles of more than 30,000 genes from NRVCMs that were subjected to early hypoxia revealed 630 genes that were differentially regulated. The intracellular Na+ overload and Ca2+ handling genes with at least two-fold changes were confirmed. The levels of Ca2+-handling proteins (calsequestrin, calmodulin, and calreticulin), ion channels (NCX, Na+–K+-ATPase, SERCA2a, and PLB), and stress markers (RyR2, ANP, and BNP) were significantly altered in early hypoxia. These results demonstrate that early hypoxia alters Ca2+-related gene expression in NRVCMs, leading to pathological status.