Worldwide invasion routes of the pinewood nematode: What can we infer from population genetics analyses?
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- 作者：Sophie Mallez (1) (2) (3)
Chantal Castagnone (1) (2) (3)
Margarida Espada (4)
Paulo Vieira (4)
Jonathan D. Eisenback (5)
Mark Harrell (6)
Manuel Mota (4)
Takuya Aikawa (7)
Mitsuteru Akiba (8)
Hajime Kosaka (9)
Philippe Castagnone-Sereno (1) (2) (3)
Thomas Guillemaud (1) (2) (3)
1. UMR 1355 Institut Sophia Agrobiotech ; INRA ; 06903 ; Sophia Antipolis ; France
2. UMR Institut Sophia Agrobiotech ; Universit茅 de Nice Sophia Antipolis ; 06903 ; Sophia Antipolis Cedex ; France
3. UMR 7254 Institut Sophia Agrobiotech ; CNRS ; 06903 ; Sophia Antipolis Cedex ; France
4. NemaLab/ICAAM 鈥?Instituto de Ci锚ncias Agr谩rias e Ambientais Mediterr芒nicas ; Universidade de 脡vora ; N煤cleo da Mitra ; Ap. 94 ; 7002-554 ; 脡vora ; Portugal
5. Department of Plant Pathology ; Physiology ; and Weed Science ; Virginia Tech ; Blacksburg ; VA ; 24061 ; USA
6. Nebraska Forest Service ; University of Nebraska ; Lincoln ; NE ; 68583-0815 ; USA
7. Tohoku Research Center ; Forestry and Forest Products Research Institute ; Morioka ; Iwate ; 020-0123 ; Japan
8. Forest Pathology Laboratory ; Forestry and Forest Products Research Institute ; Matsunosato 1 ; Tsukuba ; Ibaraki ; 305-8687 ; Japan
9. Kyushu Research Center ; Forestry and Forest Products Research Institute ; Kurokami 4-11-16 ; Kumamoto ; 860-0862 ; Japan - 关键词：Bursaphelenchus xylophilus ; Population genetics ; Diversity ; Genetic structure ; Microsatellite markers ; Pine wilt disease
- 刊名：Biological Invasions
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：17
- 期：4
- 页码：1199-1213
- 全文大小：4,003 KB
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- 刊物主题：Life Sciences
Plant Sciences
Ecology
Hydrobiology
Zoology
Forestry - 出版者：Springer Netherlands
- ISSN：1573-1464
文摘
Identifying the invasion routes and determining the origin of new outbreaks of invasive species are of crucial importance if we are to understand the invasion process, improve or establish regulatory measures and, potentially, limit the damage. We focused here on the invasion of Europe by the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner & Buhrer, 1934; Nickle 1970; Nematoda: Aphelenchoididae), a major pest of forest ecosystems, native to North America and already invasive in Asia since the beginning of the twentieth century. We evaluated the genetic diversity and structure of worldwide field PWN samples by classical and Bayesian population genetics methods to determine the source of the European invasive populations and the number of introduction events in Europe. We found (1) a very strong spatial genetic structure in native PWN populations, (2) a very low level of polymorphism in each of the invaded areas and (3) contrasted results concerning the origin of European invasive populations. Our findings provide evidence for: (1) a large effect of genetic drift on the biological cycle of the PWN, due to intense demographic bottlenecks during tree infections, not compensated for by effective dispersal of its vector; (2) a single introduction event for each of the invaded areas in Japan and Europe and a small effective size for the introduced populations and (3) a mainland Portuguese origin for PWN populations from Madeira. However, more sophisticated methods of invasion route inference and broader sampling are required to conclusively determine the origin of the European outbreak.