Improved growth rate in Clostridium thermocellum hydrogenase mutant via perturbed sulfur metabolism
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- 作者：Ranjita Biswas ; Charlotte M. Wilson ; Richard J. Giannone…
- 关键词：Cellulosic ethanol ; Clostridium thermocellum ; Redox balance ; Metabolic engineering ; Sulfate reduction
- 刊名：Biotechnology for Biofuels
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：1630KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
- 出版者：BioMed Central
- ISSN：1754-6834
- 卷排序：10
文摘
Background Metabolic engineering is a commonly used approach to develop organisms for an industrial function, but engineering aimed at improving one phenotype can negatively impact other phenotypes. This lack of robustness can prove problematic. Cellulolytic bacterium Clostridium thermocellum is able to rapidly ferment cellulose to ethanol and other products. Recently, genes involved in H2 production, including the hydrogenase maturase hydG and NiFe hydrogenase ech, were deleted from the chromosome of C. thermocellum. While ethanol yield increased, the growth rate of ΔhydG decreased substantially compared to wild type.