Engineering Yarrowia lipolytica for production of medium-chain fatty acids

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• 作者：Charles D. Rutter ; Shuyan Zhang…
• 关键词：Oleaginous yeast ; Medium ; chain fatty acids ; Acyl ; ACP thioesterase ; Metabolic engineering
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：99
• 期：17
• 页码：7359-7368
• 全文大小：1,029 KB
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• 作者单位：Charles D. Rutter (1)
  Shuyan Zhang (1)
  Christopher V. Rao (1)
  
  1. Department of Chemical and Biomolecular Engineering, University of Illinois-Urbana Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Lipids are naturally derived products that offer an attractive, renewable alternative to petroleum-based hydrocarbons. While naturally produced long-chain fatty acids can replace some petroleum analogs, medium-chain fatty acid would more closely match the desired physical and chemical properties of currently employed petroleum products. In this study, we engineered Yarrowia lipolytica, an oleaginous yeast that naturally produces lipids at high titers, to produce medium-chain fatty acids. Five different acyl-acyl carrier protein (ACP) thioesterases with specificity for medium-chain acyl-ACP molecules were expressed in Y. lipolytica, resulting in formation of either decanoic or octanoic acid. These novel fatty acid products were found to comprise up to 40?% of the total cell lipids. Furthermore, the reduction in chain length resulted in a twofold increase in specific lipid productivity in these engineered strains. The medium-chain fatty acids were found to be incorporated into all lipid classes.