Verification of serological relationship between two phylogenetically related peanut-infecting Tospovirus species

详细信息    查看全文

• 作者：Ya-Chi Kang (1) (2)
  Shyi-Dong Yeh (2) (3)
  Chih-Hsuan Liao (1)
  Wan-Chen Chou (1)
  Fang-Lin Liu (2)
  Jia-Hong Dong (4)
  Tsung-Chi Chen (1) (5)
  
• 关键词：Tospovirus ; Nucleocapsid protein ; Antibody ; Serogroup
• 刊名：European Journal of Plant Pathology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：140
• 期：4
• 页码：815-828
• 全文大小：1,503 KB
• 参考文献：1. Chen, C. C., & Chiu, R. J. (1996). A tospovirus infecting peanut in Taiwan. / Acta Horticulturae, 431, 57-7.
  2. Chen, C. C., Chen, T. C., Lin, Y. H., Yeh, S. D., & Hsu, H. T. (2005a). A chlorotic spot disease on calla lilies ( / Zantedeschia spp.) is caused by a tospovirus serologically but distantly related to Watermelon silver mottle virus. / Plant Disease, 89, 440-45. CrossRef
  3. Chen, T. C., Hsu, H. T., Jain, R. K., Huang, C. W., Lin, C. H., Liu, F. L., et al. (2005b). Purification and serological analyses of tospoviral nucleocapsid proteins expressed by / Zucchini yellow mosaic virus vector in squash. / Journal of Virological Methods, 129, 113-24. CrossRef
  4. Chen, T. C., Huang, C. W., Kuo, Y. W., Liu, F. L., Hsuan Yuan, C. H., Hsu, H. T., et al. (2006). Identification of common epitopes on a conserved region of NSs proteins among tospoviruses of / Watermelon silver mottle virus serogroup. / Phytopathology, 96, 1296-304. CrossRef
  5. Chen, T. C., Lu, Y. Y., Cheng, Y. H., Chang, C. A., & Yeh, S. D. (2008). / Melon yellow spot virus in watermelon: a first record from Taiwan. / Plant Pathology, 57, 765. CrossRef
  6. Chen, T. C., Lu, Y. Y., Cheng, Y. H., Li, J. T., Yeh, Y. C., Kang, Y. C., et al. (2010). Serological relationship between melon yellow spot virus and / Watermelon silver mottle virus and differential detection of the two viruses in cucurbits. / Archives of Virology, 155, 1085-095. CrossRef
  7. Chen, T. C., Lu, Y. Y., Kang, Y. C., Li, J. T., Yeh, Y. C., Kormelink, R., et al. (2011). Detection of eight different / Tospovirus species by a monoclonal antibody against the common epitope of NSs protein. / Acta Horticulturae, 901, 61-6.
  8. Chu, F. H., Chao, C. H., Peng, Y. C., Lin, S. S., Chen, C. C., & Yeh, S. D. (2001). Serological and molecular characterization of / Peanut chlorotic fan- / spot virus, a new species of the genus / Tospovirus. Phytopathology, 91, 856-63. CrossRef
  9. Ciuffo, M., Tavella, L., Pacifico, D., Masenga, V., & Turina, M. (2008). A member of a new / Tospovirus species isolated in Italy from wild buckwheat ( / Polygonum convolvulus). / Archives of Virology, 153, 2059-068. CrossRef
  10. Ciuffo, M., Kurowski, C., Vivoda, E., Copes, B., Masenga, V., Falk, B. W., et al. (2009). A new / Tospovirus sp. in cucurbit crops in Mexico. / Plant Disease, 93, 467-74. CrossRef
  11. Cortes, I., Livieratos, I. C., Derks, A., Peters, D., & Kormelink, R. (1998). Molecular and serological characterization of / Iris yellow spot virus, a new and distinct tospovirus species. / Phytopathology, 88, 1276-282. CrossRef
  12. Dayhoff, M. O., Schwartz, R. M., &?Orcutt, B. C. (1979). In / Atlas of protein sequence and structure, Vol. 5, pp. 345-52. Ed M.O. Dayhoff. National Biomedical Research Foundation: Silver Spring.
  13. de ávila, A. C., Huguenot, C., Resende, R. O., Kitajima, E. W., Goldbach, R. W., & Peters, D. (1990). Serological differentiation of 20 isolates of tomato spotted wilt virus. / Journal of General Virology, 71, 2801-807. CrossRef
  14. de Avila, A. C., de Haan, P., Kormelink, R., Resende, R. O., Goldbach, R. W., & Peters, D. (1993). Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. / Journal of General Virology, 74, 153-59. CrossRef
  15. de Haan, P., Wagemakers, L., Peters, D., & Goldbach, R. (1990). The S RNA segment of tomato spotted wilt virus has an ambisense character. / Journal of General Virology, 71, 1001-007. CrossRef
  1
• 作者单位：Ya-Chi Kang (1) (2)
  Shyi-Dong Yeh (2) (3)
  Chih-Hsuan Liao (1)
  Wan-Chen Chou (1)
  Fang-Lin Liu (2)
  Jia-Hong Dong (4)
  Tsung-Chi Chen (1) (5)
  
  1. Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan
  2. Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan
  3. NCHU-UCD Plant and Food Biotechnology Program, Biotechnology Center, National Chung Hsing University, Taichung, Taiwan
  4. Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China
  5. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan
  
• ISSN：1573-8469

文摘

Based on the serological relationships of nucleocapsid proteins (NPs), a tospovirus species can be classified as a member of a serogroup or a distinct serotype, which greatly helps virus identification and disease diagnosis. Recent studies reported that distinct tospovirus species sharing above 51.8?% amino acid (aa) identity in their NPs may be serologically related. Two phylogenetically related peanut-infecting tospovirus species, Peanut chlorotic fan-spot virus (PCFV) in Taiwan and Peanut yellow spot virus (PYSV) in India, were previously considered as distinct serotypes, since no serological relationship has been established. To verify the serological relationship of PCFV and PYSV, the NP of PCFV was purified from leaf tissues of the infected Chenopodium quinoa plants and used to produce polyclonal antiserum (RAs-PCFV NP) and a monoclonal antibody (MAb-PCFV NP). Polyclonal antiserum to the bacterially expressed NP of PYSV (RAs-PYSV NP) was also prepared. RAs-PCFV NP reacted with the homologous PCFV NP and the bacterial-expressed PYSV NP and tissue extracts of PYSV-infected plants. Reciprocally, RAs-PYSV NP reacted with the homologous bacterial-expressed PYSV NP and the tissue extracts of PYSV-infected or PCFV-infected plants. In addition, MAb-PCFV NP reacted only with the tissue extracts of PCFV-infected plants. Our results demonstrate that PCFV NP is serologically related to PYSV NP and they should be classified as members of a unique serogroup.