Resistance spectrum assay and fine mapping of the blast resistance gene from a rice experimental line, IRBLta2-Re
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- 作者：Shen Chen (1) (2) (3)
Jing Su (2) (3)
Jingluan Han (2) (3)
Wenjuan Wang (2) (3)
Congying Wang (2) (3)
Jianyuan Yang (2) (3)
Liexian Zeng (2) (3)
Xiaojing Wang (1)
Xiaoyuan Zhu (2) (3)
Chengwei Yang (1) - 关键词：Rice blast ; Resistance spectrum (RS) ; Resistance gene ; Fine mapping ; Single amino acid difference
- 刊名：Euphytica
- 出版年：2014
- 出版时间：January 2014
- 年：2014
- 卷：195
- 期：2
- 页码：209-216
- 全文大小：
- 作者单位：Shen Chen (1) (2) (3)
Jing Su (2) (3)
Jingluan Han (2) (3)
Wenjuan Wang (2) (3)
Congying Wang (2) (3)
Jianyuan Yang (2) (3)
Liexian Zeng (2) (3)
Xiaojing Wang (1)
Xiaoyuan Zhu (2) (3)
Chengwei Yang (1)
1. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, 510631, China
2. The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China
3. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China - ISSN：1573-5060
文摘
In the present study, we performed the resistance assessment by rice blast inoculation on IRBLta2-Re and IRBLta-CP1, the experimental lines supposed to carry rice blast resistance genes Pita2 and Pita, respectively. The analysis by using 196 rice blast isolates derived from China indicated that the resistance spectrum of IRBLta2-Re was broader than that of IRBLta-CP1. Both IRBLta2-Re and IRBLta-CP1 have the Pita gene by analyzing the functional single amino acid difference of Pita/pita locus. To identify the additional gene in IRBLta2-Re, 1250 F2 individuals from the cross between CO39 and IRBLta2-Re were used as the mapping population. The F2 population was inoculated with the blast isolate 08-T4 which was incompatible to IRBLta2-Re, but compatible to CO39 and IRBLta-CP1. In the phenotypic data analysis, the F2 population segregated in a 3:1 ratio for resistant and susceptible plants, respectively, suggesting that IRBLta2-Re has an additional resistance gene other than Pita, which was tentatively designated Pita3(t) (supposed to be Pita2). To identify the Pita3(t), a total of 50 microsatellite and 12 position specific microsatellite markers distributed by two sides of the Pita gene were selected in the parent polymorphism screening. The results showed that PT4 and PT5 were co-segregated with the target gene. A contig map corresponding to the resistance gene and Pita genes was constructed based on the fine mapping and bioinformatics assay. The resistance gene, Pita3(t), was, thus, assumed to be in an interval of approximately 178kb which containing a total of 5 NBSRR genes, and was about 500kb away from the Pita gene.