Cell-free DNA in the plasma of patients with systemic sclerosis
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- 作者：Marta Mosca ; Tiziana Giuliano ; Giovanna Cuomo ; Marica Doveri ; Chiara Tani ; Michele Curcio ; Giuseppina Abignano ; Francesca De Feo ; Laura Bazzichi and Alessandra Della Rossa ; et al.
- 关键词：Biomarkers ; Cell ; free DNA ; Disease activity ; Plasma cell ; free DNA ; Scleroderma ; Systemic autoimmune diseases
- 刊名：Clinical Rheumatology
- 出版年：2009
- 出版时间：December, 2009
- 年：2009
- 卷：28
- 期：12
- 页码：1437-1440
- 全文大小：88.4 KB
文摘
The aim of the present study was to evaluate the concentration of cell-free DNA (cf-DNA) in the plasma of patients with systemic sclerosis (SSc) and to examine the correlation of cf-DNA with clinical variables of the disease. The study population consisted of 122 SSc patients and 16 healthy controls. Epidemiological and clinical data were collected by direct assessment. The β-globin gene was used to determine the total amount of DNA in the plasma by real-time quantitative PCR analysis. cf-DNA was found in all patients (mean concentration 1,420.7 copies/ml) and controls (mean concentration 1,462.5), with no significant difference. In SSc patients, no correlation was found between cf-DNA and the type of organ involvement, but patients with active disease presented significantly higher cf-DNA concentrations than those with inactive disease (p < 0.05). Our data suggest that cf-DNA could provide a useful biomarker for the assessment of disease activity in SSc patients.