Thrombomodulin mediates the migration of cervical cancer cells through the regulation of epithelial–mesenchymal transition biomarkers

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• 作者：Cheng-Jeng Tai (1) (2)
  Chao-Wen Cheng (3)
  Hou-Yu Su (4)
  Wei-Yu Chen (5)
  Chun-Te Wu (6) (7)
  Feng-Yen Lin (2) (8)
  Chien-Kai Wang (1) (10) (2) (9)
  Chen-Jei Tai (10) (9)
  Po-Li Wei (11) (12) (13)
  
• 关键词：Thrombomodulin ; Cervical cancer ; Migration ; EMT
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：35
• 期：1
• 页码：47-54
• 全文大小：542 KB
• 作者单位：Cheng-Jeng Tai (1) (2)
  Chao-Wen Cheng (3)
  Hou-Yu Su (4)
  Wei-Yu Chen (5)
  Chun-Te Wu (6) (7)
  Feng-Yen Lin (2) (8)
  Chien-Kai Wang (1) (10) (2) (9)
  Chen-Jei Tai (10) (9)
  Po-Li Wei (11) (12) (13)
  
  1. Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, Republic of China
  2. Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, Republic of China
  3. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, Republic of China
  4. Department of Surgery, Saint Mary’s Hospital Luodong, Yilan County, Taiwan, Republic of China
  5. Department of Pathology, Wan-Fang Hospital and Taipei Medical University, Taipei, Taiwan, Republic of China
  6. Chang Gung University College of Medicine, Taipei, Taiwan, Republic of China
  7. Department of Urology, Chang Gung Memorial Hospital at Keelung, Keelung, Taiwan, Republic of China
  8. Division of Cardiology, Taipei Medical University Hospital, Taipei, Taiwan, Republic of China
  10. Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, Republic of China
  9. Department of Chinese Medicine, Taipei Medical University Hospital, Taipei, Taiwan, Republic of China
  11. Department of Surgery, College of Medicine, Taipei Medical University, Taipei, Taiwan, Republic of China
  12. Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan, Republic of China
  13. Cancer Research Center, Taipei Medical University Hospital, 250 Wu-Xin Street, Taipei, Taiwan, Republic of China
  
• ISSN：1423-0380

文摘

Thrombomodulin (TM) has been shown to regulate many physiological and pathological processes, including inflammation, thrombosis, and tumor progression. TM is also a natural anticoagulant that maintains circulatory homeostasis in endothelial cells. However, little is known regarding the role of TM in the progression and metastasis of cervical cancer. TM-specific RNA interference and a cDNA expression vector were used to manipulate TM expression in cervical cancer cells. Cell growth and cell migration were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transwell migration assays, and a biosensor system. TM silencing did not affect the growth rate of the cells. However, cell migration was dramatically enhanced after silencing of TM in HeLa cells. The overexpression of TM in cervical cancer cells only slightly influenced their proliferative capacity. After overexpression of TM in HeLa cells, their migratory capability was suppressed. Furthermore, we found that the decreased expression of E-cadherin and increase of zeb-1 and snail expression in TM-silenced cells which may be correlated with the results of knocking-down TM increases the migratory ability in this study. Our results demonstrate that TM may slightly regulate the growth but played the important role in the migratory ability of cervical cancer cells, suggesting that TM could potentially serve as a novel prognostic and therapeutic target in cervical cancer.