The phylogenetic and recombinational analysis of beak and feather disease virus Taiwan isolates

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• 作者：Shr-Wei Huang ; Yu-Chung Chiang ; Chien-Yu Chin ; Pin-Chi Tang…
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：161
• 期：11
• 页码：2969-2988
• 全文大小：16,309 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798
• 卷排序：161

文摘

Beak and feather disease virus (BFDV) is an avian circovirus, and it has a single-stranded DNA genome. It causes a fatal disease in parrots called psittacine beak and feather disease (PBFD). After screening of samples collected from Taiwan using PCR, complete genome sequences of isolates from 21 samples from various species of parrot were obtained. The nucleotide sequences of the replication-associated protein gene (rep) and the amino acid sequences of the replication-associated protein (Rep) were more conserved than the nucleotide sequences of the capsid protein gene (cp) and the amino acid sequences of the capsid protein (CP). In Bayesian phylogenetic analysis, the topology of the complete genome sequence was similar to that of the rep gene alone. Recombination events were identified in Taiwan isolates. Recombination hot spots were mainly located in the intergenic region between the 3’ ends of the rep and cp genes and at the 5’ end of the cp gene. The 5’ end and the middle of the rep gene were found to be recombination cold spots. Despite the overall negative selection that was observed for the rep and cp genes, one and 18 positive selected sites were found for the rep and cp gene, respectively.