Synthesis of boronic-acid-functionalized magnetic attapulgite for selective enrichment of nucleosides

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• 作者：Ting Cheng ; Huihui Li ; Ying Ma ; Xiaoyan Liu…
• 关键词：Boronate affinity chromatography ; Nucleosides ; Magnetic ; Attapulgite ; Enrichment
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：407
• 期：12
• 页码：3525-3529
• 全文大小：435 KB
• 参考文献：1. James, TD, Sandanayake, KRAS, Shinka, S (1996) Saccharide sensing with molecular receptors based on boronic acid. Angew Chem Int Ed Engl 35: pp. 1910-1922 CrossRef
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

2,4-Difluoro-3-formyl-phenylboronic acid (DFFPBA)-modified magnetic attapulgite (ATP-Fe3O4-NH2-DFFPBA) was synthesized and employed to capture and enrich cis-diol-containing biomolecules. The resulting material exhibited a high saturation magnetization value of 20.71?emu/g, allowing the absorbent to be conveniently magnetically separated. Combining the Fe3O4 nanoparticles with the high specific surface area of attapulgite yielded a material with a high capture capacity (13.78?mg/g) for adenosine. Furthermore, ATP-Fe3O4-NH2-DFFPBA was found to possess remarkable selectivity for adenosine at a low molar ratio of adenosine/2-deoxyadenosine (1:500). The potential applications of this material were explored by using it to extract five nucleosides from urine samples, and the results demonstrate that it can decrease matrix interference and selectively enrich analytes. Graphical Abstract Boronic-acid-functionalized magnetic attapulgite could selectively enrich the nucleosides in urine samples with the help of an external magnet