RFLP) in 302 cases and 316 controls. Results showed that individuals with the rs6421571CT and TT genotypes had a strong correlation with the incidence of CRC (odds ratio (OR)--.46; 95?% confidence interval (CI), 1.02-.09; p--.041 and OR--.62; 95?% CI, 1.50-.95; p--.001, respectively). Also, rs80202369AA genotype revealed significantly higher distribution in CRC patients than in controls (p--.002). We further investigated the possible effects of the polymorphisms by assessing messenger RNA (mRNA) and protein levels of CXCR5 in peripheral blood mononuclear cells (PBMCs), CD4+ T cells, CD8+ T cells, and B cells. Data presented that healthy controls with rs6421571CT and TT genotypes had higher mRNA and protein levels of CXCR5 than those with wild-type CC genotype specifically in CD4+ T cells. These findings suggest novel risk factors of CRC and indicate a potential mechanism of CXCR5 gene polymorphism." />
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C-X-C chemokine receptor type 5 gene polymorphism affects gene expression in CD4+ T cells and is associated with increased risk of colorectal cancer
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  • 作者：Junjie Xing ; Xu Li ; Jinke Sui ; Guangwen Cao ; Chuangang Fu
  • 关键词：C ; X ; C chemokine receptor type 5 ; Polymorphisms ; Colorectal cancer
  • 刊名：Tumor Biology
  • 出版年：2014
  • 出版时间：August 2014
  • 年：2014
  • 卷：35
  • 期：8
  • 页码：7929-7934
  • 全文大小：308 KB
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    8. Li Y, Ma S, Tang L, Li Y, Wang W, et al. Circulating chemokine (C-X-C Motif) receptor 5(+) CD4(+) T cells benefit hepatitis B e antigen seroconversion through IL-21 in patients with chronic hepatitis B virus infection. Hepatology. 2013;58:1277-6. CrossRef
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    16. Pallikkuth S, Parmigiani A, Silva SY, George VK, Fischl M, et al. Impaired peripheral blood T-follicular helper cell function in HIV-infected nonresponders to the 2009 H1N1/09 vaccine. Blood. 2012;120:985-3. CrossRef
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  • 作者单位：Junjie Xing (1)
    Xu Li (1)
    Jinke Sui (1)
    Guangwen Cao (2)
    Chuangang Fu (1)

    1. Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China
    2. Department of Epidemiology, Second Military Medical University, 800 Xiangyin Rd., Shanghai, 200433, China
  • ISSN：1423-0380
文摘
Dysregulation of the immune system may play important roles in the development of colorectal cancer (CRC). The C-X-C chemokine receptor type 5 (CXCR5) is one of the principal regulators for targeting T cells, B cells, and dendritic cells into secondary lymphoid organs. The current study investigated the association between CXCR5 gene polymorphisms and the risk of CRC, and the potential effect of these polymorphisms on different immune cells. Two polymorphisms in CXCR5 gene, rs6421571C/T and rs80202369G/A, were examined by polymerase chain reaction–restriction fragment length polymorphism (PCR-a href='/search?dc.title=RFLP&facet-content-type=ReferenceWorkEntry&sortOrder=relevance' class='reference-link webtrekk-track' gaCategory="Internal link" gaLabel="RFLP" gaAction="reference keyword">RFLP) in 302 cases and 316 controls. Results showed that individuals with the rs6421571CT and TT genotypes had a strong correlation with the incidence of CRC (odds ratio (OR)--.46; 95?% confidence interval (CI), 1.02-.09; p--.041 and OR--.62; 95?% CI, 1.50-.95; p--.001, respectively). Also, rs80202369AA genotype revealed significantly higher distribution in CRC patients than in controls (p--.002). We further investigated the possible effects of the polymorphisms by assessing messenger RNA (mRNA) and protein levels of CXCR5 in peripheral blood mononuclear cells (PBMCs), CD4+ T cells, CD8+ T cells, and B cells. Data presented that healthy controls with rs6421571CT and TT genotypes had higher mRNA and protein levels of CXCR5 than those with wild-type CC genotype specifically in CD4+ T cells. These findings suggest novel risk factors of CRC and indicate a potential mechanism of CXCR5 gene polymorphism.

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