Identification of differentially expressed genes between hepatocytes of Landes geese (Anser anser) and Sichuan White geese (Anser cygnoides)
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- 作者：Zhixiong Pan ; Jiwen Wang ; Chunchun Han ; Ningfeng Zhai ; Jia Lv ; Zehui Zhou ; Hui Tang ; Shuxia Xiang ; Jing Wang and Liang Li
- 关键词：Goose primary hepatocytes ; Suppression subtractive hybridization (SSH) ; Real ; time quantitative PCR ; Differential expression ; Expressed sequence tags (ESTs)
- 刊名：Molecular Biology Reports
- 出版年：2010
- 出版时间：December 2010
- 年：2010
- 卷：37
- 期：8
- 页码：4059-4066
- 全文大小：202.1 KB
文摘
In this study, we examined gene expression in order to identify genes that are differentially expressed between the hepatocytes of Sichuan White geese and Landes geese. We hypothesized that such genes may be involved in the different predispositions between these two species to develop hepatic steatosis. RNA was isolated from primary hepatocytes of the two species, and suppression subtractive hybridization was employed to screen for genes that showed differences in mRNA expression. We built and characterized two reciprocal cDNA libraries that were enriched in genes up-regulated in Landes geese or Sichuan White geese. Using dot blot analysis we identified 128 of 600 randomly selected sequences that demonstrated differential expression between the two species. Of these differentially expressed genes, 115 sequences shared high homology with 46 known genes and 13 sequences corresponded to eight novel expressed sequence tags (ESTs). Based on functional clustering, up and down-regulated genes were mostly related to lipid metabolism, nuclear mRNA splicing, enzyme activity and transcription control. The expression of 18 selected clones was further studied by quantitative PCR. The data showed that eight clones similar to the genes ACSL5, CTGF, CIDEA, PPARγ, PCK, GSTS1, RPS4X, and THBS1 had significantly higher expression levels in the hepatocytes of Landes geese. In contrast, seven clones similar to the genes ADH5, YBX1, ASAH1, UCB, AOPVLDL, SCD-1, and ELOVL-6 had significantly higher expression levels in the hepatocytes of Sichuan White geese.