Use of site-specific DNA endonucleases in genome-wide studies of human DNA

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• 作者：M. A. Abdurashitov ; S. Kh. Degtyarev
• 关键词：site ; specific endonucleases ; genomics ; epigenomics ; DNA methylation ; sequencing
• 刊名：Russian Journal of Genetics
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：53
• 期：1
• 页码：1-8
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics;
• 出版者：Pleiades Publishing
• ISSN：1608-3369
• 卷排序：53

文摘

During the last decades, site-specific DNA endonucleases have served as a key instrument to study primary structure of DNA and genetic engineering. Here, we describe examples of these enzyme uses in genome-wide analysis of human DNA including restriction endonucleases involvement during sample preparation for sequencing using NGS devices, as well as visualization of cleavage of DNA repeats by endonucleases. The first studies on application of DNA endonucleases in the rapidly developing area of epigenetic analysis of genomes, which is facilitated by the recent discovery of a new class of enzymes, 5-methylcytosinedependent site-specific DNA endonucleases, are of special interest.