In Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagen

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• 作者：Esma Eryilmaz ; Winfried Teizer ; Wonmuk Hwang
• 关键词：AFM ; Biomimetic surface ; Collagen ; Extracellular matrix ; Heterogeneous assembly ; Macromolecular assembly
• 刊名：Cellular and Molecular Bioengineering
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：10
• 期：1
• 页码：41-53
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering; Classical Mechanics; Continuum Mechanics and Mechanics of Materials; Biological and Medical Physics, Biophysics; Biomaterials; Cell Biology;
• 出版者：Springer US
• ISSN：1865-5033
• 卷排序：10

文摘

An important step towards achieving functional diversity of biomimetic surfaces is to better understand the co-assembly of the extracellular matrix components. For this, we study type-I and type-III collagen, the two major collagen types in the extracellular matrix. By using atomic force microscopy, custom image analysis, and kinetic modeling, we study their homotypic and heterotypic assembly. We find that the growth rate and thickness of heterotypic fibrils decrease as the fraction of type-III collagen increases, but the fibril nucleation rate is maximal at an intermediate fraction of type-III. This is because the more hydrophobic type-I collagen nucleates fast and grows in both longitudinal and lateral directions, whereas more hydrophilic type-III limits lateral growth of fibrils, driving more monomers to nucleate additional fibrils. This demonstrates that subtle differences in physico-chemical properties of similar molecules can be used to fine-tune their assembly behavior.