Microbial Community Composition and Extracellular Enzyme Activities Associated with Juncus roemerianus and Spartina alterniflora Vegetated Sediments in Louisiana Saltmarshes

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• 作者：Anthony J. Rietl ; Megan E. Overlander ; Andrew J. Nyman…
• 关键词：Microbial diversity ; Extracellular enzyme activity ; Wetland microbial communities ; Saltmarshes
• 刊名：Microbial Ecology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：71
• 期：2
• 页码：290-303
• 全文大小：677 KB
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• 作者单位：Anthony J. Rietl (1)
  Megan E. Overlander (2)
  Andrew J. Nyman (1)
  Colin R. Jackson (2)
  
  1. School of Renewable Natural Resources, Renewable Natural Resources Department, Louisiana State University, Baton Rouge, LA, 70803, USA
  2. Department of Biology, The University of Mississippi, Shoemaker Hall, Oxford, MS, 38677, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Ecology
  Geoecology and Natural Processes
  Nature Conservation
  
• 出版者：Springer New York
• ISSN：1432-184X

文摘

Saltmarshes are typically dominated by perennial grasses with large underground rhizome systems that can change local sediment conditions and be important in shaping the sediment microbial community. Factors such as salinity that control plant zonation in saltmarshes are also likely to influence the microbial community, but little is known as to whether microbial communities share distribution patterns with plants in these systems. To determine the extent to which microbial assemblages are influenced by saltmarsh plant communities, as well as to examine patterns in microbial community structure at local and regional scales, we sampled sediments at three saltmarshes in Louisiana, USA. All three systems exhibit a patchy distribution of Juncus roemerianus stands within a Spartina alterniflora marsh. Sediment samples were collected from the interior of several J. roemerianus stands as well as from the S. alterniflora matrix. Samples were assayed for extracellular enzyme activity and DNA extracted to determine microbial community composition. Denaturing gradient gel electrophoresis of rRNA gene fragments was used to determine regional patterns in bacterial, archaeal, and fungal assemblages, while Illumina sequencing was used to examine local, vegetation-driven, patterns in community structure at one site. Both enzyme activity and microbial community structure were primarily influenced by regional site. Within individual saltmarshes, bacterial and archaeal communities differed between J. roemerianus and S. alterniflora vegetated sediments, while fungal communities did not. These results highlight the importance of the plant community in shaping the sediment microbial community in saltmarshes but also demonstrate that regional scale factors are at least as important. Keywords Microbial diversity Extracellular enzyme activity Wetland microbial communities Saltmarshes