Bacillus amyloliquefaciens T-5 may prevent Ralstonia solanacearum infection through competitive exclusion

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• 作者：Shiyong Tan ; Yian Gu ; Chunlan Yang ; Yue Dong ; Xinlan Mei…
• 关键词：Biological control ; CLSM ; Fluorescent protein ; Root colonization ; Tomato
• 刊名：Biology and Fertility of Soils
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：52
• 期：3
• 页码：341-351
• 全文大小：731 KB
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• 作者单位：Shiyong Tan (1) (2) (3) (4)
  Yian Gu (1) (3)
  Chunlan Yang (1) (3)
  Yue Dong (1) (3)
  Xinlan Mei (1) (3)
  Qirong Shen (1) (3)
  Yangchun Xu (1) (3)
  
  1. National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing, 210095, China
  2. Key Laboratory of Plant Nutrition and Biological Fertilizer, Ministry of Agriculture, Changsha, 410205, Hunan, People’s Republic of China
  3. Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China
  4. Hunan Taigu Biotechnology Co., Ltd, Changsha, 410205, Hunan, China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Life Sciences
  Agriculture
  Soil Science and Conservation
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0789

文摘

Investigation of the properties and mechanisms of the interactions of root-colonizing biocontrol bacteria and plant pathogens is necessary to optimize the biocontrol strategies. In the present study, the interaction of a biocontrol strain Bacillus amyloliquefaciens T-5 tagged with a green fluorescent protein marker and a bacterial wilt pathogen Ralstonia solanacearum QL-Rs1115 tagged with red fluorescent protein marker was studied on tomato roots using different inoculation methods. The results showed that in the co-culture experiment, the population of pathogen QL-RFP was decreased by increasing the initial inoculum concentration of biocontrol strain. In the greenhouse experiment, both strains T-5-GFP and QL-RFP colonized tomato roots (root tips, root hairs, primary roots, and root junctions) and formed a biofilm on the root surfaces as determined by dilution plating and confocal laser scanning microscopy (CLSM) techniques. However, the root colonization of pathogen strain QL-RFP was almost completely suppressed in the presence of biocontrol strain T-5-GFP when both soil and plant seedlings were treated with T-5-GFP. The results of this study revealed the effectiveness of strain B. amyloliquefaciens T-5 as a biocontrol agent against tomato wilt pathogen and the significance of inoculation method used to inoculate biocontrol strain.