Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

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• 作者：Noura El-Ahmady El-Naggar ; Hassan Moawad ; Nayera A. M. Abdelwahed
• 关键词：Streptomyces brollosae NEAE ; 115 ; L ; asparaginase production ; Solid state fermentation ; Soybean meal ; Plackett–Burman design and central composite design
• 刊名：Annals of Microbiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：67
• 期：1
• 页码：1-15
• 全文大小：
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Mycology; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044
• 卷排序：67

文摘

Optimization of the fermentation conditions for extracellular production of L-asparaginase by Streptomyces brollosae NEAE-115 under solid state fermentation was investigated. The Plackett–Burman experimental design was used to screen 16 independent variables (incubation time, moisture content, inoculum size, temperature, pH, soybean meal + wheat bran, dextrose, fructose, L-asparagine, yeast extract, KNO₃, K₂HPO₄, MgSO₄.7H₂O, NaCl, FeSO₄. 7H₂O, CaCl₂) and three dummy variables. The most significant independent variables found to affect enzyme production, namely soybean + wheat bran (X₆), L-asparagine (X₉) and K₂HPO₄ (X₁₂), were further optimized by the central composite design. We found that L-asparaginase production by S. brollosae NEAE-115 was 47.66, 129.92 and 145.57 units per gram dry substrate (U/gds) after an initial survey using “soybean meal + wheat bran” as a substrate for L-asparaginase production (step 1), statistical optimization by Plackett–Burman design (step 2) and further optimization by the central composite design (step 3), respectively, with a fold of increase of 3.05.