Colorectal adenoma and carcinoma specific miRNA profiles in biopsy and their expression in plasma specimens
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- 作者：Zsófia Brigitta Nagy ; Barnabás Wichmann ; Alexandra Kalmár…
- 关键词：microRNA ; Colorectal cancer ; Colorectal adenoma ; miRNA profiling ; Microarray ; Real ; time PCR
- 刊名：Clinical Epigenetics
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：9
- 期：1
- 全文大小：1887KB
- 刊物主题：Human Genetics; Gene Function;
- 出版者：BioMed Central
- ISSN：1868-7083
- 卷排序：9
文摘
BackgroundMiRNA expression markers are well characterized in colorectal cancer (CRC), but less is known about miRNA expression profiles in colorectal adenomas. Genome-wide miRNA and mRNA expression analyses were conducted through the colorectal adenoma dysplasia sequence. Furthermore, analysis of the expression levels of miRNAs in matched plasma samples was performed, focusing on biomarker candidates; miRNA and mRNA expression analyses were performed on colorectal biopsies and plasma samples (20 normals; 11 tubular and 9 tubulovillous adenomas; 20 colorectal carcinomas) by miRNA 3.0 and Human Transcriptome Array (Affymetrix) and validated by RT-qPCR. Microarray data were analyzed using Expression Console and mRNA targets were predicted using miRWALK 2.0.