Molecular and cellular response of earthworm Eisenia andrei (Oligochaeta, Lumbricidae) to PCDD/Fs exposure
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- 作者：Shreen Deeb Nusair ; Yousef Sa’id Abu Zarour
- 关键词：Earthworms ; Eisenia worms ; Dioxins ; Furans ; Proteins ; Catalase ; Histology ; Acute toxicity tests
- 刊名：Environmental Science and Pollution Research
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：24
- 期：1
- 页码：902-910
- 全文大小：
- 刊物类别：Earth and Environmental Science
- 刊物主题：Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water M
- 出版者：Springer Berlin Heidelberg
- ISSN：1614-7499
- 卷排序：24
文摘
The acute toxicity of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) was investigated in the earthworm Eisenia andrei using filter paper toxicity test. Protein content, catalase (CAT) activity, and histology of intestinal wall (chloragogen cells and intestinal epithelium) were investigated in earthworms exposed for 48 h to 0 (control), 0.5, 1.0, and 1.5 ng/cm2 PCDD/Fs. The results showed an increase in the total protein content 1.56- (p = 0.104), 1.66- (p = 0.042), and 2.26-fold (p < 0.001), respectively, compared to control. The average ± standard deviation of tissular CAT activity showed no significant differences; it was 36.01 ± 7.65, 36.17 ± 9.45, 36.08 ± 9.80, and 40.01 ± 6.98 U/g tissue, respectively. However, the average specific activity of CAT ± standard deviation was significantly decreased (p < 0.001) at all doses compared to control; it was 2.93 ± 0.42, 1.93 ± 0.53, 1.80 ± 0.38, and 1.53 ± 0.44 U/mg protein, respectively. There was a progressive damage in both of the intestinal villi and the chloragogenous tissue associated with the incrementing doses. Since the toxic mixture altered the investigated biomarkers of E. andrei within 48 h, the cellular and molecular alterations resulted from the filter paper contact test could be utilized as a rapid toxicity assessment tool of environmental contamination with dioxins/furans and to assess consequent potential adverse effects on soil biota and other organisms in the ecosystem.