Overexpression of ScALDH21 gene in cotton improves drought tolerance and growth in greenhouse and field conditions

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• 作者：Honglan Yang ; Daoyuan Zhang ; Xiaoshuang Li ; Haiyan Li ; Dawei Zhang…
• 关键词：Syntrichia caninervis ; ScALDH21 ; Transgenic cotton ; Drought tolerance ; Cotton yield
• 刊名：Molecular Breeding
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：36
• 期：3
• 全文大小：1,375 KB
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• 作者单位：Honglan Yang (1) (2)
  Daoyuan Zhang (1)
  Xiaoshuang Li (1)
  Haiyan Li (1)
  Dawei Zhang (4)
  Haiyan Lan (3)
  Andrew J. Wood (5)
  Jiancheng Wang (1)
  
  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Xinjiang, 830011, Urumqi, China
  2. College of Resource and Environment Sciences, Xinjiang University, Urumqi, 830046, China
  4. Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China
  3. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830046, China
  5. Department of Plant Biology, Southern Illinois University-Carbondale, Carbondale, IL, 62901-6509, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Aldehyde dehydrogenase (ALDH) is essential for scavenging redundant aldehydes when plants are exposed to stress. The aim of the present study was to validate the ectopic expression of the ScALDH21 gene, which is isolated from Syntrichia caninervis, an extremely drought-tolerant moss, to improve drought tolerance in cotton (Gossypium hirsutum L.). In our study, the ScALDH21-transformed cotton was identified via PCR, RT-PCR, and DNA gel blotting, and the growth and physiological characteristics related to drought tolerance were compared between the transgenic cotton (TC) and non-transgenic cotton (NT) grown in a greenhouse and in field conditions. The results indicated that TC accumulated approximately 11.8–304 % more proline than did NT under drought stress, and produced a lower concentration of lipid peroxidation-derived reactive aldehydes and had a higher peroxidase activity under oxidative stress. Moreover, TC showed reduced loss of the net photosynthetic rate compared with NT. Under field conditions, TC showed greater plant height, larger bolls, and greater cotton fiber yield than NT, but no significant difference in fiber quality between TC and NT following different water-withholding treatments. These results suggest that overexpression of ScALDH21 can greatly improve the drought tolerance of cotton without reduction in yield and fiber quality.