Isolation and characterization of twenty polymorphic microsatellites for Hemibarbus labeo (Cyprinidae)

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• 作者：Cuiyun Lu ; Ying Gu ; Longwu Geng ; Lei Cheng ; Chao Li…
• 关键词：Hemibarbus labeo ; Microsatellite ; Genetic conservation
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：89-92
• 全文大小：164 KB
• 参考文献：1. Geng LW, Lu CY, Tong GX, Li C, Xu W (2012) Development and characterization of twenty microsatellite markers for the endangered fish / Luciobarbus capito. Conserv Genet Resour 4:865-67 CrossRef
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• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Hemibarbus labeo is a valuable freshwater fish species distributed in the Heilongjiang River basin in China. Wild resources have declined sharply in recent years because of overfishing and pollution. Herein, twenty polymorphic microsatellites were isolated from an enriched genomic library in H. labeo for genetic conservation. These markers were characterized in 27 wild and 48 cultured individuals. The number of alleles per locus ranged from two to ten, with an average of 5.200. The observed heterozygosity and the expected heterozygosity varied from 0.086 to 0.870 (mean?=?0.538) and from 0.116 to 0.861 (mean?=?0.5441), respectively. Three and two loci significantly deviated from Hard–Weinberg equilibrium in wild and cultured individuals, respectively. Three pairwise comparisons of six loci showed significant genotypic linkage disequilibrium in cultured individuals. These polymorphic markers represent a powerful tool to study population genetic diversity, genetic conservation and breeding systems in H. labeo.