Analysis of cylindrospermopsin- and microcystin-producing genotypes and cyanotoxin concentrations in the Macau storage reservoir
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- 作者：Weiying Zhang (1)
Inchio Lou (1)
Wai Kin Ung (2)
Yijun Kong (2)
Kai Meng Mok (1) - 关键词：pks ; mcy ; Cylindrospermopsin ; Microcystin ; Toxic cyanobacteria ; PCR and qPCR
- 刊名：Hydrobiologia
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：741
- 期：1
- 页码：51-68
- 全文大小：812 KB
- 参考文献：1. APHA, 1995. Standard Methods. 19th Edition. American Public Health Association, Washington, DC, USA.
2. Chorus, I. & J. Bartram, 1999. Toxic Cyanobacteria in Water. A Guide to their Public Health Consequences Monitoring and Management. E&FN Spon, London. CrossRef
3. Christiansen, G., J. Fastner, M. Erhard, T. B?rner & E. Dittmann, 2003. Microcystin biosynthesis in / Planktothrix: genes, evolution and manipulation. Journal of Bacteriology 185: 564-72. CrossRef
4. Conradie, K. R. & S. Barnard, 2012. The dynamics of toxic / Microcystis strains and microcystin production in two hypertrofic South African reservoirs. Harmful Algae 20: 1-0. CrossRef
5. Davis, T. W., D. L. Berry, G. L. Boyer & C. J. Gobler, 2009. The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of / Microcystis during cyanobacterial blooms. Harmful Algae 8: 715-25. CrossRef
6. Dittmann, E., B. A. Neilan, M. Erhard, H. von D?hren & T. B?rner, 1997. Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium / Microcystis aeruginosa PCC 7806. Molecular Microbiology 26: 779-87. CrossRef
7. Dolman, A. M., J. Rücker, F. R. Pick, J. Fastner, T. Rohrlack, U. Mischke & C. Wiedner, 2012. Cyanobacteria and cyanotoxins: the influence of nitrogen versus phosphorus. PLoS One 7: e38757. CrossRef
8. Duy, T. N., P. K. Lam, G. R. Shaw & D. W. Connell, 2000. Toxicology and risk assessment of freshwater cyanobacterial (blue-green algal) toxins in water. Reviews of Environmental Contamination and Toxicology 163: 113-85.
9. Eaglesham, G. K., R. L. Norris, G. R. Shaw, M. J. Smith, R. K. Chiswell, B. C. Davis, G. R. Neville, A. A. Seawright & M. R. Moore, 1999. Use of HPLC–MS/MS to monitor cylindrospermopsin, a blue-green algal toxin, for public health purposes. Environmental Toxicology 14: 151-54. CrossRef
10. Fergusson, K. M. & C. P. Saint, 2003. Multiplex PCR Assay for / Cylindrospermopsis raciborskii and cylindrospermopsin-producing cyanobacteria. Environmental Toxicology 18: 120-25. CrossRef
11. Furukawa, K., N. Noda, S. Tsuneda, T. Saito, T. Itayama & Y. Inamori, 2006. Highly sensitive real-time assay for quantification of toxic cyanobacteria based on Microcystin synthetase A gene. Journal of Bioscience and Bioengineering 102: 90-6. CrossRef
12. Herry, S. E., A. Fathalli, A. J. Rejeb & N. Bouaicha, 2008. Seasonal occurrence and toxicity of / Microcystis spp. and / Oscillatoria tenuis in the Lebna Dam, Tunisia. Water Research 42: 1263-273. CrossRef
13. Hotto, A. M., M. F. Satchwell, D. L. Berry, C. J. Gobler & G. L. Boyer, 2008. Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY. Harmful Algae 7: 671-81. CrossRef
14. Humpage, A., 2008. Toxin types, toxicokinetics and toxicodynamics. Advances in Experimental Medicine and Biology 619: 383-15. CrossRef
15. Joung, S., H. Oh, S. Ko & C. Ahn, 2011. Correlations between environmental factors and toxic and non-toxic / Microcystis dynamics during bloom in Daechung Reservoir, Korea. Harmful Algae 10: 188-93. CrossRef
16. Kaebernick, M., E. Dittmann, T. B?rner & B. A. Neilan, 2002. Multiple alternate transcripts direct the biosynthesis of microcystin, a cyanobacterial nonribosoma - 作者单位：Weiying Zhang (1)
Inchio Lou (1)
Wai Kin Ung (2)
Yijun Kong (2)
Kai Meng Mok (1)
1. Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Av. Padre Tomás Pereira Taipa, Macau, SAR, China
2. Laboratory & Research Center, Macao Water Co. Ltd., 718, Avenida do Conselheiro Borja, Macau, SAR, China - ISSN：1573-5117
文摘
The Macau storage reservoir (MSR) has experienced algal blooms in recent years, with high levels of Cylindrospermopsis and Microcystis and detectable concentrations of cyanotoxins. To analyze the cyanotoxin-producing genotypes and relate the corresponding cyanotoxins to the water quality parameters, a quantitative real-time polymerase chain reaction was developed and applied to the water samples in three locations of MSR. Cylindrospermopsin polyketide synthetase (pks) gene and a series of microcystin synthetase (mcy) genes were used for identifying and quantifying cylindrospermopsin- and microcystin-producing genes, and the corresponding water parameters were measured accordingly. Our results showed that high concentrations of cylindrospermopsin and low concentrations of microcystin were measured during the study period. There was a strong correlation between the pks gene numbers and cylindrospermopsin concentrations (R 2?=?0.95), while weak correlations were obtained between the mcy genes numbers and microcystin concentrations. Furthermore, the pks gene numbers were strongly related to Cylindrospermopsis (R 2?=?0.88), cyanobacterial cell numbers (R 2?=?0.96), total algae numbers (R 2?=?0.95), and chlorophyll-a concentrations (R 2?=?0.83), consistent with the dominant species of Cylindrospermopsis among the cyanobacteria existing in MSR. NH4–N (R 2?=?0.68) and pH (R 2?=?0.89) were the water quality parameters most highly correlated with the pks gene numbers. These results contribute to monitoring for potential cyanotoxins in raw water.