Effects of glutamate on growth, antioxidant capacity, and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes

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• 作者：Jun Jiang ; Dan Shi ; Xiao-Qiu Zhou ; Long Yin ; Lin Feng…
• 关键词：Growth ; Antioxidant capacity ; Signaling molecule ; Enterocytes ; Glutamate
• 刊名：Fish Physiology and Biochemistry
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：41
• 期：5
• 页码：1143-1153
• 全文大小：590 KB
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• 作者单位：Jun Jiang (1) (2) (3)
  Dan Shi (1) (2)
  Xiao-Qiu Zhou (2) (3)
  Long Yin (1)
  Lin Feng (2) (3)
  Yang Liu (2) (3)
  Wei-Dan Jiang (2) (3)
  Ye Zhao (1) (2)
  
  1. College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan Province, People’s Republic of China
  2. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China
  3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

The present study explored the effects of glutamate (Glu) on the growth, antioxidant capacity, and gene expression of NF-E2-related nuclear factor 2 (Nrf2) signaling molecule in enterocytes of Jian carp (Cyprinus carpio var. Jian). The enterocytes were incubated in media containing 0, 2, 4, 6, 8, and 10 mM/L Glu for 96 h. The results showed that Glu could promote fish enterocytes proliferation and differentiation. Additionally, activities of alkaline phosphatase, Na+, K+-ATPase, γ-glutamyl transpeptidase, and creatine kinase were significantly improved with the increase in Glu level up to 6 mM/L. Lactic acid dehydrogenase activity and malondialdehyde content in the medium and cellular protein carbonyls were depressed by Glu. Moreover, optimum Glu significantly enhanced glutathione content and the activities and gene expression of catalase, glutathione reductase, and glutathione peroxidase in enterocytes. Finally, the expression level of Nrf2 in enterocytes was significantly elevated by appropriate Glu content in the medium. Furthermore, optimum Glu significantly decreased Kelch-like ECH-associated protein 1 mRNA level in enterocytes. In conclusion, Glu improved the proliferation, function, and antioxidant capacity and regulated antioxidant-related signaling molecule expression of fish enterocytes. Keywords Growth Antioxidant capacity Signaling molecule Enterocytes Glutamate