Synthesis and protein binding properties of novel highly functionalized Calix[4]arene

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• 作者：Reza Zadmard ; Shukufe Darvishi…
• 关键词：Calix[4]arene ; Protein ; Fluorescence ; Lysozyme ; Binding affinity
• 刊名：Journal of Inclusion Phenomena and Macrocyclic Chemistry
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：86
• 期：1-2
• 页码：27-32
• 全文大小：776 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Organic Chemistry
  Food Science
  Crystallography
  
• 出版者：Springer Netherlands
• ISSN：1573-1111
• 卷排序：86

文摘

A novel protein binding agent based on highly functionalized calix[4]arene 5 has been synthesized and its structure characterized by 1H and 13C NMR, FT-IR and HRMS spectral data. Protein binding studies carried out using fluorescence spectroscopic technique revealed that this compound could bind strongly to lysozyme. Furthermore, the effect of compound 5 on the conformation of lysozyme was investigated using three-dimensional fluorescence spectroscopy. Finally, the influence of various biological relevant metal ions on the binding constant of lysozyme with 5 was studied.