Knockdown of Akt2 Expression by ShRNA Inhibits Proliferation, Enhances Apoptosis, and Increases Chemosensitivity to Paclitaxel in Human Colorectal Cancer Cells
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- 作者：Zhongyang Ding (1) (3)
Fei Xu (2)
Gan Li (3)
Jiandong Tang (3)
Zhangfeng Tang (3)
Panqiang Jiang (3)
Haotian Wu (3)
1. Department of Surgery ; Nanjing Medical University affiliated Wuxi People鈥檚 Hospital ; Wuxi ; 214023 ; China
3. Department of General Surgery ; Nanjing TCM University affiliated Nanjing TCM Hospital ; Wuxi ; 214042 ; China
2. Medical Examination Center ; Nanjing Medical University affiliated Wuxi Women and Children health hospital ; Wuxi ; 214153 ; China - 关键词：CRC ; Akt2 ; MDR ; 1 ; MRP ; 1
- 刊名：Cell Biochemistry and Biophysics
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：71
- 期：1
- 页码：383-388
- 全文大小：605 KB
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- 出版者：Springer US
- ISSN：1559-0283
文摘
Akt2 overexpression correlates with chemoresistance of colorectal cancer (CRC). However, the cellular functions and precise signals elicited by Akt2 in LSCC have not been elucidated. Here, we transfected a CRC cell line HCT116 with Akt-2 targeted shRNA in order to establish a cell line with Akt2 knockdown. In vitro experiments showed that knockdown Akt2 in HCT116 cells was associated with decrease in cell proliferation as well as enhanced cell apoptosis. Furthermore, our results demonstrated that Akt2 knockdown correlated with elevated chemosensitivity of HCT116 cells to paclitaxel. Importantly, we found that knockdown of AKt2 resulted in downregulation of MDR-1 and MRP-1. Our findings may lead to a better understanding of the biological effect of Akt2 and may provide mechanistic insights for developing potential therapeutic strategies targeting AKt2.