RNAi Targeting Putative Genes in Phosphatidylcholine Turnover Results in Significant Change in Fatty Acid Composition in Crambe abyssinica Seed Oil

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• 作者：Rui Guan (1)
  Xueyuan Li (1)
  Per Hofvander (1)
  Xue-Rong Zhou (2) (3)
  Danni Wang (1) (4)
  Sten Stymne (1)
  Li-Hua Zhu (1)
  
  1. Department of Plant Breeding ; Swedish University of Agricultural Sciences ; PO Box 101 ; Alnarp ; 230 53 ; Sweden
  2. Food and Nutrition and Bioproducts Flagship ; Commonwealth Scientific and Industrial Research Organisation (CSIRO) ; PO Box 1600 ; Canberra ; ACT ; 2601 ; Australia
  3. Agriculture Flagship ; Commonwealth Scientific and Industrial Research Organisation (CSIRO) ; PO Box 1600 ; Canberra ; ACT ; 2601 ; Australia
  4. Department of Nutrition ; Food and Exercise Sciences ; Florida State University ; Tallahassee ; FL ; 32306-1493 ; USA
  
• 关键词：Crambe abyssinica ; LPCAT ; PDCT ; PDAT ; RNAi ; Fatty acid composition
• 刊名：Lipids
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：50
• 期：4
• 页码：407-416
• 全文大小：1,035 KB
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  35. Guan R,聽Lager I,聽Li X,聽Stymne S, Zhu LH (2014) Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid聽 / Crambe abyssinica.聽Plant Biotechnol J聽12:193鈥?03. http://dx.doi.org/10.1111/pbi.12128
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307

文摘

The aim of this study was to evaluate the importance of three enzymes, LPCAT, PDCT and PDAT, involved in acyl turnover in phosphatidylcholine in order to explore the possibility of further increasing erucic acid (22:1) content in Crambe seed oil. The complete coding sequences of LPCAT1-1 and LPCAT1-2 encoding lysophosphatidylcholine acyltransferase (LPCAT), PDCT1 and PDCT2 encoding phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), and PDAT encoding phospholipid:diacylglycerol acyltransferase (PDAT) were cloned from developing Crambe seeds. The alignment of deduced amino acid sequences displayed a high similarity to the Arabidopsis homologs. Transgenic lines expressing RNA interference (RNAi) targeting either single or double genes showed significant changes in the fatty acid composition of seed oil. An increase in oleic acid (18:1) was observed, to varying degrees, in all of the transgenic lines, and a cumulative effect of increased 18:1 was shown in the LPCAT-PDCT double-gene RNAi. However, LPCAT single-gene RNAi led to a decrease in 22:1 accumulation, while PDCT or PDAT single-gene RNAi had no obvious effect on the level of 22:1. In agreement with the abovementioned oil phenotypes, the transcript levels of the target genes in these transgenic lines were generally reduced compared to wild-type levels. In this paper, we discuss the potential to further increase the 22:1 content in Crambe seed oil through downregulation of these genes in combination with fatty acid elongase and desaturases.