Optical diagnosis of gallbladder cancers via two-photon excited fluorescence imaging of unstained histological sections

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• 作者：Zhipeng Hong (1) (2)
  Youting Chen (2)
  Jing Chen (1)
  Hong Chen (3)
  Yahao Xu (1)
  Xiaoqin Zhu (1) (4)
  Shuangmu Zhuo (1)
  Zheng Shi (2)
  Jianxin Chen (1)
  
  1. Institute of Laser and Optoelectronics Technology ; Fujian Provincial Key Laboratory for Photonics Technology ; Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education ; Fujian Normal University ; Fuzhou ; 350007 ; People鈥檚 Republic of China
  2. Department of Hepatopancreatobiliary Surgery and Institute of Abdominal Surgery ; The First Affiliated Hospital of Fujian Medical University ; Fuzhou ; 350005 ; People鈥檚 Republic of China
  3. Department of Pathology ; The First Affiliated Hospital of Fujian Medical University ; Fuzhou ; 350005 ; People鈥檚 Republic of China
  4. Beckman Laser Institute and Medical Clinic ; University of California ; Irvine ; California 92612 ; USA
  
• 关键词：Gallbladder cancers ; Diagnosis ; Unstained histological sections ; Two ; photon excited fluorescence imaging ; Pathology
• 刊名：Lasers in Medical Science
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：30
• 期：1
• 页码：225-233
• 全文大小：17,760 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Medicine/Public Health, general
  Dentistry
  Laser Technology and Physics and Photonics
  Quantum Optics, Quantum Electronics and Nonlinear Optics
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer London
• ISSN：1435-604X

文摘

Two-photon excited fluorescence (TPEF) microscopy, based on signal from cells, can provide detailed information on tissue architecture and cellular morphology in unstained histological sections to generate subcellular-resolution images from tissue directly. In this paper, we used TPEF microscopy to image microstructure of human normal gallbladder and three types of differentiated carcinomas in order to investigate the morphological changes of tissue structure, cell, cytoplasm, and nucleus without hematoxylin and eosin (H&E) staining. It displayed that TPEF microscopy can well image the stratified normal gallbladder tissue, including the mucosa, the muscularis, and the serosa. The typical cancer cell, characterized by cellular and nuclear pleomorphism, enlarged nuclei, and augmented nucleolus, can be identified in histological sections without H-E staining as well. The quantitative results showed that the areas of the nucleus and the nucleolus in three types of cancerous cells were all significantly greater than those in normal gallbladder columnar epithelial cells derived from TPEF microscopic images. The studies demonstrated that TPEF microscopy has the ability to characterize tissue structures and cell morphology of gallbladder cancers differentiated from a normal gallbladder in a manner similar to traditional histological analysis. As a novel tool, it has the potential for future retrospective studies of tumor staging and migration by utilizing histological section specimens without H-E staining.