Development of a new method for analyzing free aluminum ions (Al3+) in seafood using HPLC-ICP-MS

详细信息    查看全文

• 作者：DeRong Shang (1)
  YanFang Zhao (1)
  YuXiu Zhai (1)
  JinSong Ning (1)
  HaiYan Ding (1)
  XiaoFeng Sheng (1)
  
• 关键词：free aluminum ion ; seafood ; solid ; phase extraction ; HPLC ; ICP ; MS
• 刊名：Chinese Science Bulletin
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：58
• 期：35
• 页码：4437-4442
• 全文大小：
• 作者单位：DeRong Shang (1)
  YanFang Zhao (1)
  YuXiu Zhai (1)
  JinSong Ning (1)
  HaiYan Ding (1)
  XiaoFeng Sheng (1)
  
  1. Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China
  
• ISSN：1861-9541

文摘

This paper presents a novel method for simultaneous online examination of free aluminum ions (Al3+) in seafood, using solid-phase extraction and high-performance liquid chromatography online with inductively coupled plasma mass spectrometry (SPE-HPLC-ICP-MS), without post-column reaction. The optimum conditions for chromatographic separation of Al3+ were achieved using an IonPac CS5A analytical column with an IonPac CG5A guard column. The mobile phase consisted of 0.040 mol/L LiOH, 0.0060 mol/L 2,6-pyridinedicarboxylic acid, and 0.090 mol/L CH3COOH (pH 4.7). The free Al3+ ions in seafood were extracted by shaking with the mobile phase at 70°C for 2 h. SPE was conducted using an Oasis MCX, 3cc/60 mg, 30 μm column, which was activated and equilibrated with 2 mL of methanol and 4 mL of deionized water before use. HCl (0.075 mol/L, 2 mL) was used to wash inorganic Al from the SPE column. The standard recoveries of Al3+ were all above 89% and the relative standard deviations were all below 5%. The proposed method was successfully used for the examination of Al3+ in seafood samples, and the results were similar to those obtained using the static equilibrium method.