Biochemical characterization of laccase from hairy root culture of Brassica juncea L. and role of redox mediators to enhance its potential for the decolorization of textile dyes
详细信息 查看全文
- 作者：Amar A. Telke (1)
Anuradha N. Kagalkar (2)
Umesh B. Jagtap (3)
Neetin S. Desai (4)
Vishwas A. Bapat (3)
Sanjay P. Govindwar (2) - 关键词：Brassica ; Decolorization ; Hairy roots ; Laccase ; Phytoremediation ; Redox mediators
- 刊名：Planta
- 出版年：2011
- 出版时间：December 2011
- 年：2011
- 卷：234
- 期：6
- 页码：1137-1149
- 全文大小：545KB
- 参考文献：1. Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gubitz GM (2000) Decolourization and detoxification of textile dyes with a laccase from / Trametes hirsute. Appl Environ Microb 66:3357-362 CrossRef
2. Agostini E, Coniglio MS, Milrad SR, Tigier HA, Giulietti AM (2003) Phytoremediation of 2,4-dichlorophenol by / Brassica napus hairy root cultures. Biotechnol Appl Biochem 37:139-44 CrossRef
3. Ajungla L, Patil PP, Barmukh RB, Nikam TD (2009) Influence of biotic and abiotic elicitors on accumulation of hyoscyamine and scopolamine in root cultures of / Datura metel L. Indian J Biotechnol 8:317-22
4. Arthur EL, Rice PJ, Anderson TA, Baladi SM, Henderson KLD, Coats IR (2005) Phytoremediation––an overview. Crit Rev Plant Sci 24:109-22 CrossRef
5. Baldrian P (2004) Purification and characterization of laccase from the white rot fungus / Daedalea quercina and decolorization of synthetic dyes by the enzyme. Appl Microbiol Biotechnol 63:560-63 CrossRef
6. Baldrian P (2006) Fungal laccases: occurrence and properties. FEMS Microbiol Rev 30:215-42 CrossRef
7. Bizani E, Fytianos K, Poulios I, Tsiridis V (2006) Photocatalytic decolorization and degradation of dye solutions and wastewaters in the presence of titanium dioxide. J Hazard Mater 136:85-4 CrossRef
8. Call H, Mucke I (1997) Minireview: history, overview and applications of mediated ligninolytic systems, especially laccase-mediator-systems (Lignozym-Process). J Biotechnol 53:163-02 CrossRef
9. Chhabra M, Mishra S, Sreekrishnan T (2009) Laccase/mediator assisted degradation of triarylmethane dyes in a continuous membrane reactor. J Biotechnol 43:69-8 CrossRef
10. Claus H (2004) Laccases: structure, reactions, distribution. Micron 35:93-6 CrossRef
11. Claus H, Faber G, K?nig H (2002) Redox-mediated decolorization of synthetic dyes by fungal laccases. Appl Microbiol Biotechnol 59:672-78 CrossRef
12. Crini G (2006) Non-conventional low-cost adsorbents for dye removal: a review. Bioresour Technol 97:1061-085 CrossRef
13. Doran PM (2009) Applications of plant tissue cultures in phytoremediation research: incentives and limitations. Biotechnol Bioeng 103:60-6 CrossRef
14. Duckworth HW, Coleman JE (1970) Physicochemical and kinetic properties of mushroom tyrosinase. J Biol Chem 245:1613-625
15. Ghodake G, Telke A, Jadhav J, Govindwar S (2009) Potential of / Brassica juncea in order to treat textile effluent contaminated sites. Int J Phytorem 11:297-12 CrossRef
16. Jadhav SU, Ghodake GS, Telke AA, Tamboli DP, Govindwar SP (2009) Degradation and detoxification of disperse dye Scarlet RR by / Galactomyces geotrichum MTCC 1360. J Microbiol Biotechnol 19:409-15 CrossRef
17. Joo DJ, Shin WS, Choi JH, Choi SJ, Kim MC, Han MH, Ha TW, Kim YH (2007) Decolorization of reactive dyes using inorganic coagulants and synthetic polymer. Dyes Pigment 73:59-4 CrossRef
18. Kagalkar AN, Jagtap UB, Jadhav JP, Bapat VA, Govindwar SP (2009) Biotechnological strategies for phytoremediation of the sulfonated azo dye direct red 5B using / Blumea malcolmii Hook. Bioresour Technol 100:4104-110 CrossRef
19. Kagalkar AN, Jagtap UB, Jadhav JP, Bapat VA, Govindwar SP (2010) Studies on phytoremediation potentiality of / Typhonium flagelliforme for the degradation of Brilliant Blue R. Planta 232:271-85 CrossRef
20. Kalme S, Jadhav S, Jadhav M, Govindwar S (2009) Textile dye degrading laccase from / Pseudomonas desmolyticum NCIM 2112. Enzym Microb Technol 44:65-1 CrossRef
21. Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-75
22. Mathur N, Bhatnagar P, Bakre P (2005) Assessing mutagenicity of textile dyes from Pali (Rajasthan) using Ame’s bioassay. Appl Ecol Environ Res 4:111-18
23. Moldes D, Sanroman MA (2006) Amelioration of the ability to decolorize dyes by laccase: relationship between redox mediators and laccase isoenzymes in / Trametes vericolor. World J Microbiol Biotechnol 22:1197-204 CrossRef
24. Morozova OV, Shumakovich GP, Shleev SV, Yaropolov YI (2007) Laccase–mediator systems and their applications: a review. Appl Biochem Microbiol 43:523-35 CrossRef
25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15:473-79 CrossRef
26. Murugesan K, Arulmani M, Nam IH, Kim YM, Chang YS, Kalaichelvan PT (2006) Purification and characterization of laccase produced by a white rot fungus / Pleurotus sajorcaju under submerged culture condition and its potential in decolorization of azo dyes. Appl Microbiol Biotechnol 72:939-46 CrossRef
27. Nagai M, Sato T, Watanabe H, Saito K, Kawata M, Enei H (2002) Purification and characterization of an extracellular laccase from the edible mushroom / Lentinula edodes and decolorization of chemically different dyes. Appl Microbiol Biotechnol 60:327-35 CrossRef
28. Newman L, Reynolds C (2004) Phytodegradation of organic compounds. Curr Opin Biotech 15:225-30
29. Patil P, Desai N, Govindwar S, Jadhav J, Bapat V (2009) Degradation analysis of reactive red 198 by hairy roots of / Tagetes patula L (Marigold). Planta 230:725-35 CrossRef
30. Sharma P, Goel R, Capalash N (2007) Bacterial laccases. World J Microbiol Biotechnol 23:823-32 CrossRef
31. Sharma S, Singh P, Swami R, Sharma K (2009) Exploring fish bioassay of textile dye wastewaters and their selected constituents in terms of mortality and erythrocyte disorders. Bull Environ Contam Toxicol 83:29-4 CrossRef
32. Singh S, Melo JS, Eapen S, D’Souza SF (2006) Phenol removal using / Brassica juncea hairy roots: role of inherent peroxidase and H2O2. J Biotechnol 123:43-9 CrossRef
33. Sterjiades R, Dean J, Eriksson K (1992) Laccase from Sycamore maple ( / Acer pseudoplatanus) polymerizes monolignols. Plant Physiol 99:1162-168 CrossRef
34. Suresh B, Sherkhane PD, Kale S, Eapen S, Ravishankar GA (2005) Uptake and degradation of DDT by hairy root cultures of / Cichorium intybus and / Brassica juncea. Chemosphere 61:1288-292 CrossRef
35. Switzer RC, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231-37 CrossRef
36. Telke AA, Kalyani DC, Dawkar VV, Govindwar SP (2009a) Influence of organic and inorganic compounds on oxidoreductive decolorization of sulfonated azo dye C.I. Reactive orange 16. J Hazard Mater 172:298-09 CrossRef
37. Telke AA, Kalyani DC, Jadhav UV, Parshetti GK, Govindwar SP (2009b) Purification and characterization of an extracellular laccase from a / Pseudomonas sp. / LBC1. J Mol Catal B Enzym 61:252-60 CrossRef
38. Telke AA, Kadam AA, Jagtap SS, Jadhav JP, Govindwar SP (2010) Biochemical characterization of blue laccase from / Aspergillus ochraceus NCIM-1146 and its potential for textile dye degradation. Biotechnol Biopro Eng 15:696-03
39. Tien M, Kirk T (1983) Lignin-degrading enzyme from the hymenomycete / Phanerochaete chrysosporium burds. Science 221:661-63 CrossRef
40. Xu F (1997) Effects of redox potential and hydroxide inhibition on the pH activity profile of fungal laccases. J Biol Chem 272:924-28
41. Yun M, Yeon K, Park J, Lee C, Chun J, Lim D (2006) Characterization of biofilm structure and its effect on membrane permeability in MBR for dye wastewater treatment. Water Res 40:45-2 CrossRef
42. Zille A, Tzanov T, Gübitz GM, Cavaco-Paulo M (2003) Immobilized laccase for decolourization of reactive black 5 dyeing effluent. Biotechnol Lett 25:1473-477 CrossRef - 作者单位：Amar A. Telke (1)
Anuradha N. Kagalkar (2)
Umesh B. Jagtap (3)
Neetin S. Desai (4)
Vishwas A. Bapat (3)
Sanjay P. Govindwar (2)
1. Synthetic Biotechnology Laboratory, Division of Applied Life Sciences, Gyeongsang National University, Jinju, Korea Republic
2. Department of Biochemistry, Shivaji University, Kolhapur, 416004, India
3. Department of Biotechnology, Shivaji University, Kolhapur, 416004, India
4. Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, Sect-15/50 C. B. D. Belapur, Navi Mumbai, 400614, India
文摘
In vitro transgenic hairy root cultures provide a rapid system for physiological, biochemical studies and screening of plants for their phytoremediation potential. The hairy root cultures of Brassica juncea L. showed 92% decolorization of Methyl orange within 4?days. Out of the different redox mediators that were used to achieve enhanced decolorization, 2, 2-Azinobis, 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) was found to be the most efficient. Laccase activity of 4.5?U?mg? of protein was observed in hairy root cultures of Brassica juncea L., after the decolorization of Methyl orange. Intracellular laccase produced by B. juncea root cultures grown in MS basal medium was purified up to 2.0 fold with 6.62?U?mg? specific activity using anion-exchange chromatography. Molecular weight of the purified laccase was estimated to be 148?kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified enzyme efficiently oxidized ABTS which was also required for oxidation of the other tested substrates. The pH and temperature optimum for laccase activity were 4.0 and 40°C, respectively. The purified enzyme was stable up to 50°C and was stable in the pH range of 4.0-.0. Laccase activity was strongly inhibited by sodium azide, EDTA, dithiothreitol and l-cysteine. The purified enzyme decolorized various textile dyes in the presence of ABTS as an efficient redox mediator. These findings contribute to a better understanding of the enzymatic process involved in phytoremediation of textile dyes by using hairy roots.