Identification and expression profiling of DNA methyltransferases during development and stress conditions in Solanaceae
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- 作者：Rahul Kumar ; Pankaj Kumar Chauhan ; Ashima Khurana
- 关键词：DNA methyltransferase ; Solanum lineage ; Transcript profiling ; Gene family ; Tomato
- 刊名：Functional & Integrative Genomics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：16
- 期：5
- 页码：513-528
- 全文大小：9,634 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Plant Genetics and Genomics
Microbial Genetics and Genomics
Biochemistry
Bioinformatics
Animal Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1438-7948
- 卷排序：16
文摘
DNA methyltransferase (DMTase) enzymes contribute to plant development and stress responses by de novo establishment and subsequent maintenance of DNA methylation during replication. However, the molecular mechanism underlying this activity remains obscure, especially in crop species. Using DMTase homolog complement in six Solanaceae species, we demonstrated here that their number remained conserved in Solanum lineage, whereas it was expanded in both pepper and Nicotiana benthamiana. Non-synonymous vs synonymous (Ka/Ks) substitution ratio revealed that most of the Solanaceous DMTase homologs undergo purifying selection. The genomic sequences of tomato DMT homologs in its wild relative, Solanum pennellii, remained highly conserved in their exons and methyltransferase domains. Structure analysis further revealed highly similar folding of DMTase homologs and conservation in the residues participating in protein-protein interaction in Solanum lineage, whereas a considerable diversification was observed of pepper homologs. Transcript profiling of DMTases highlighted both similar and distinct expression patterns of tomato homologs in other species during fruit development and stress responses. Overall, our analysis provides a strong basis for in-depth exploration of both conserved as well as distinct functions of tomato DMTase homologs in other economically important Solanaceae species.