Development of a Detection Kit Based on G-Quadruplex DNAzyme for Detection of Lead(II) Ion in Food Samples

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• 作者：Tao Liu ; Gang Nie ; Xiao Zhang ; Wei Liu ; Wentao Zhang…
• 关键词：Lead(II) ion ; Kit ; Colorimetric method ; DNAzyme ; Guaiacol
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：8
• 期：5
• 页码：1133-1140
• 全文大小：1,331 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

The biocatalytic function of G-quadruplex DNAzyme has been first used to design a detection kit for colorimetric detection of Pb2+ in food samples by coupling guaiacol (GA) as an effective and ideal colorimetric signal indicator. Addition of Pb2+ enabled the G-rich DNA to bind hemin to fabricate the Pb2+-promoted DNAzyme, which can catalyze the H2O2-mediated oxidation of colorless GA to produce amber tetraguaiacol, causing a visible color change. Both standard curve and colorimetric card were constructed in the present detection kit for the quantitative analysis of Pb2+. Under optimum conditions, the working range of the kit was 10-00?nM with a detection limit of 1?nM Pb2+. And the performance of the colorimetric card method of the kit could discriminate Pb2+ from 5 to 80?nM. The shelf-life of the kit was 6?months at 4?°C, and the effectiveness and reliability of the kit on the determination of Pb2+ were evaluated in real food samples. Therefore, the good performance characteristics of the DNAzyme-based detection kit indicate that it holds a promising application for sensitive and low-cost detection of Pb2+ ions in foods.