Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed
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- 作者：Lingling An ; Yulian Wang ; Yuanhu Pan ; Yanfei Tao ; Dongmei Chen…
- 刊名：Food Analytical Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：9
- 期：9
- 页码：2434-2443
- 全文大小：494 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Food Science
Chemistry
Microbiology
Analytical Chemistry - 出版者：Springer New York
- ISSN：1936-976X
- 卷排序：9
文摘
To monitor the illegal use of florfenicol (FF) and thiamphenicol (TAP) in edible animal tissue and feed, a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed with simple sample preparation and cleanup. The obtained monoclonal antibody (5F4) that has isotype IgG1 showed an IC50 value of 0.21 μg L−1 for FF and 0.35 μg L−1 for TAP, respectively. It did not exhibit measurable cross-reactivity with other antibiotics. The limits of detection (LODs) for FF and TAP in a muscle matrix ranged from 0.07 to 0.14 μg kg−1 and in a feed matrix ranged from 2.9 to 5.2 μg kg−1. The recoveries were 72.8 to 113.4 % with a coefficient of variation of less than 15 %. Good correlation between the ELISA and HPLC-MS/MS results in the tissues tested demonstrated the reliability of our ic-ELISA. This ELISA is a useful tool for screening FF and TAP in edible animal tissue and feed.KeywordsFlorfenicolThiamphenicolIndirect competitive enzyme-linked immunosorbent assayEdible animal tissueFeed