Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester
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- 作者：Xu Liu (1)
Rong Chen (1) (2)
Zhongwei Yang (1)
Jiale Wang (1)
Jinping Lin (1)
Dongzhi Wei (1) - 关键词：Gluconobacter oxydans ; Oxidoreductase ; Ethyl (R) ; 4 ; chloro ; 3 ; hydroxybutanoate ester
- 刊名：Molecular Biotechnology
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：56
- 期：4
- 页码：285-295
- 全文大小：821 KB
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Rong Chen (1) (2)
Zhongwei Yang (1)
Jiale Wang (1)
Jinping Lin (1)
Dongzhi Wei (1)
1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, P.O. Box 311#, 130 Meilong Road, Shanghai, 200237, People’s Republic of China
2. Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou, 310012, China - ISSN：1559-0305
文摘
A gene encoding an NADH-dependent short-chain dehydrogenase/reductase (gox2036) from Gluconobacter oxydans 621H was cloned and heterogeneously expressed in Escherichia coli. The protein (Gox2036) was purified to homogeneity and biochemically characterized. Gox2036 was a homotetramer with a subunit size of approximately 28?kDa. Gox2036 had a strict requirement for NAD+/NADH as the cofactor. Gox2036 displayed preference for oxidation of secondary alcohols and 2,3-diols as well as for reduction of α-diketones, hydroxy ketones, α-ketoesters, and β-ketoesters. However, Gox2036 was poorly active on 1,2-diols and acetoin and showed no activity on primary alcohols, polyols, and aldehydes. The optimum pH values for the oxidation and reduction reactions were 9 and 6, respectively. Gox2036 was highly selective in the reduction of various β-ketones and β-ketoesters. Among the substrates tested, ethyl 4-chloro acetoacetate was reduced to ethyl (R)-4-chloro-3-hydroxybutanoate ester with an excellent conversion yield of 96.9?% and optical purity of >99?% e.e. using an efficient in situ NADH-recycling system involving glucose and a glucose dehydrogenase from Bacillus subtilis (BsGDH).