Eurycoma longifolia upregulates osteoprotegerin gene expression in androgen- deficient osteoporosis rat model

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• 作者：Ahmad Nazrun Shuid (1)
  Eman El-arabi (1)
  Nadia Mohd Effendy (1)
  Halimaton Saadiah Abdul Razak (2)
  Norliza Muhammad (1)
  Norazlina Mohamed (1)
  Ima Nirwana Soelaiman (1)
  
• 关键词：Eurycoma longifolia ; Osteoporosis ; Orchiectomy ; OPG ; RANKL
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：12
• 期：1
• 全文大小：588KB
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  52. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/152/prepub
  
• 作者单位：Ahmad Nazrun Shuid (1)
  Eman El-arabi (1)
  Nadia Mohd Effendy (1)
  Halimaton Saadiah Abdul Razak (2)
  Norliza Muhammad (1)
  Norazlina Mohamed (1)
  Ima Nirwana Soelaiman (1)
  
  1. Department of Pharmacology, Faculty of Medicine, National University of Malaysia (Universiti Kebangsaan Malaysia), Jalan Raja Muda Abd Aziz, 50300, KL, Kragujevac, Malaysia
  2. Department of Biomedical Science, Faculty of Health Sciences, National University of Malaysia (Universiti Kebangsaan Malaysia), Jalan Raja Muda Abd Aziz, 50300, KL, Kragujevac, Malaysia
  

文摘

Background Eurycoma longifolia (EL) has been shown recently to protect against bone calcium loss in orchidectomised rats, the model for androgen-deficient osteoporosis. The mechanism behind this is unclear but it may be related to its ability to elevate testosterone levels or it may directly affect bone remodeling. The aim of this study is to determine the mechanism involved by investigating the effects of EL extract on serum testosterone levels, bone biomarkers, biomechanical strength and gene expression of Receptor Activator of Nuclear Factor kappa-B ligand (RANKL), Osteoprotegerin (OPG) and Macrophage-Colony Stimulating Factor (MCSF) in orchidectomised rats. Methods Thirty-two male Sprague–Dawley rats were divided into: Sham-operated group (SHAM); orchidectomised-control group (ORX); orchidectomised and given 15?mg/kg EL extract (ORX + EL) and orchidectomised and given 8?mg/kg testosterone (ORX + T). The rats were treated for 6?weeks. The serum levels of testosterone, osteocalcin and C-terminal telopeptide of type I collagen (CTX) were measured using the ELISA technique. The femoral bones were subjected to biomechanical testing. The tibial bone gene expressions of RANKL, OPG and MCSF were measured using the branch DNA technique. Results The post-treatment level of testosterone was found to be significantly reduced by orchiectomy (p < 0.05). Both ORX + EL and ORX + T groups have significantly higher post-treatment testosterone levels compared to their pre-treatment levels (p < 0.05). The bone resorption marker (CTx) was elevated after orchiectomy but was suppressed after treatment in the ORX + EL and ORX + T groups (p < 0.05). There was no significant finding for the femoral biomechanical parameters. The tibial OPG gene expression in the ORX group was significantly lower compared to the SHAM and ORX + EL groups (p < 0.05). Conclusion Supplementation with EL extract elevated the testosterone levels, reduced the bone resorption marker and upregulated OPG gene expression of the orchidectomised rats. These actions may be responsible for the protective effects of EL extract against bone resorption due to androgen deficiency.