Ethanol and isopropanol trigger rapid egress of intracellular Eimeria tenella sporozoites

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• 作者：Xinlei Yan ; Xianyong Liu ; Yongsheng Ji ; Geru Tao ; Xun Suo
• 关键词：Eimeria tenella ; Egress ; Ethanol ; Isopropanol
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：114
• 期：2
• 页码：625-630
• 全文大小：2,985 KB
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• 作者单位：Xinlei Yan (1) (2)
  Xianyong Liu (1) (2) (3)
  Yongsheng Ji (1) (2)
  Geru Tao (1) (2)
  Xun Suo (1) (2) (3)
  
  1. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China
  2. National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China
  3. Key Laboratory of Zoonosis of Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Egress from host cells is a vital step of the intracellular life cycle of apicomplexan parasites such as Toxoplasma gondii. This phenomenon has attracted attentions from many research groups. Previous studies have shown that ethanol could stimulate the release of microneme proteins by elevating intracellular Ca2+ concentration of T. gondii, resulting in the parasite egress from host cells. However, little information about egress is known on Eimeria species, the causative agent of coccidiosis in poultry and livestock. In this report, we studied the effect of ethanol and isopropanol on the egress of eimerian parasites. Eimeria tenella sporozoites cultured in primary chicken kidney cells were treated with ethanol and isopropanol, then the egressed parasites were analyzed. Ethanol and isopropanol could induce the rapid egress of E. tenella sporozoites from host cells. No substantial damage was found in parasite-egressed host cells. Compared to the freshly isolated sporozoites, the re-invading ability and reproductivity of the egressed parasites significantly decreased by 43.4 and 44.1?% individually. We also found that fewer sporozoites egressed from host cells when the parasites developed for a longer time before the alcohol treatment. These results demonstrate an in vitro egress mode different from that of T. gondii, facilitating the deciphering of the mechanisms of egress of eimerian parasites.