Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein

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• 作者：Zhiwei Lin ; Yanyun Shi ; Bin Deng ; Xiangfei Mao ; Dongyou Yu…
• 关键词：Bacillus subtilis ; Chickens ; Eimeria tenella ; Oral immunization ; 3 ; 1E protein
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：114
• 期：9
• 页码：3229-3236
• 全文大小：694 KB
• 参考文献：Abi-Ghanem D, Waghela SD, Caldwell DJ, Danforth HD, Berghman LR (2008) Phage display selection and characterization of single-chain recombinant antibodies against Eimeria tenella sporozoites. Vet Immunol Immunopathol 121:58-7View Article PubMed
  Chapman HD, Shirley MW (2003) The Houghton strain of Eimeria tenella: a review of the type strain selected for genome sequencing. Avian Pathol 32:115-27View Article PubMed
  Choi KD, Lillehoj HS, Zalenga DS (1999) Changes in local IFN-gamma and TGF-beta4 mRNA expression and intraepithelial lymphocytes following Eimeria acervulina infection. Vet Immunol Immunopathol 71(3-):263-75View Article PubMed
  Cornelissen JB, Swinkels WJ, Boersma WA, Rebel JM (2009) Host response to simultaneous infections with Eimeria acervulina, maxima and tenella: a cumulation of single responses. Vet Parasitol 162(1-):58-6View Article PubMed
  Dalloul RA, Lillehoj HS (2006) Poultry coccidiosis: recent advancements in control measures and vaccine development. Expert Rev Vaccines 5(1):143-63View Article PubMed
  Dalloul RA, Lillehoj HS, Shellem TA, Doerr JA (2003) Enhanced mucosal immunity against Eimeria acervulina in broilers fed a Lactobacillus-based probiotic. Poultry Sci 82(1):62-6View Article
  Ferreira LC, Ferreira RC, Schumann W (2005) Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors. Ann Acad Bras Cienc 77:113-24
  Fu LL, Li WF, Du HH, Dai W, Xu ZR (2008) Oral vaccination with envelope protein VP28 against white spot syndrome virus in Procambarus clarkii using Bacillus subtilis as delivery vehicles. Lett Appl Microbial 46(5):581-86View Article
  Fu LL, Wang Y, Wu ZC, Li WF (2011) In vivo assessment for oral delivery of Bacillus subtilis harboring a viral protein (VP28) against white spot syndrome virus in Litopenaeus vannamei. Aquaculture 322-23:33-8View Article
  Heydari A, Moosazadeh Moghaddam M, Aghamollaei H, Yakhchali M, Bambaee B, Latifi A (2013) Cloning and expression of the Bacillus pumilus F3 lipase gene into Bacillus subtilis and determining of comparative expression level between native and recombinant enzyme. New cell Mol Biotech J 3(9):67-3
  Inagaki-Ohara K, Dewi FN, Hisaeda H, Smith A, Jimi F, Miyahira M, Abdel-Aleem AS, Horii Y, Nawa Y (2006) Intestinal intraepithelial lymphocytes sustain the epithelial barrier function against Eimeria vermiformis infection. Infect Immun 74(9):5292-301PubMed Central View Article PubMed
  Jang SI, Lillehoj HS, Lee SH, Lee KW, Park MS, Bauchan GR, Lillehoj EP, Bertrand F, Dupuis L, Deville S (2010) Immunoenhancing effects of Montanide?ISA oil-based adjuvants on recombinant coccidia antigen vaccination against Eimeria acervulina infection. Vet Parasitol 172(3-):221-28View Article PubMed
  Jang SI, Lillehoj HS, Lee SH, Lee KW, Lillehoj EP, Bertrand F, Dupuis L, Deville S (2011) Mucosal immunity against Eimeria acervulina infection in broiler chickens following oral immunization with profilin in Montanide?adjuvants. Exp Parasitol 129(1):36-1View Article PubMed
  Johnson J, Reid WM (1970) Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol 28(1):30-6View Article PubMed
  Lee SH, Lillehoj HS, Dalloul RA, Park DW, Hong YH, Lin JJ (2007) Influence of Pediococcus-based probiotic on coccidiosis in broiler chickens. Poultry Sci 86(1):63-6View Article
  Lee SH, Lillehoj HS, Park DW, Jang SI, Morales A, Garcia D, Lucio E, Larios R, Victoria G, Marrufo D, Lillehoj EP (2009) Protective effect of hyperimmune egg yolk IgY antibodies against Eimeria tenella and Eimeria maxima infections. Vet Parasitol 163:123-26View Article PubMed
  Lee S, Belitsky BR, Brinker JP, Kerstein KO, Brown DW, Clements JD, Keusch GT, Tzipori S, Sonenshein AL, Herrmann JE (2010a) Development of a Bacillus subtilis-based rotavirus vaccine. Clin Vaccine Immunol 17:1647-655PubMed Central View Article PubMed
  Lee SH, Lillehoj HS, Jang SI, Lee KW, Yancey RJ, Dominowski P (2010b) The effects of a novel adjuvant complex/Eimeria profilin vaccine on the intestinal host immune response against live E. acervulina challenge infection. Vaccine 28(39):6498-504View Article PubMed
  Lillehoj HS, Lillehoj EP (2000) Avian coccidiosis. A review of acquired intestinal immunity and vaccination strategies. Avian Dis 44(2):408-25View Article PubMed
  Lillehoj HS, Ruff MD (1987) Comparison of disease susceptibility and subclass-specific antibody response in SC and FP chickens experimentally inoculated with Eimeria tenella, E. acervulina, or E. maxima. Avian Dis 31(1):112-19View Article PubMed
  Lillehoj HS, Min W, Dalloul RA (2004) Recent progress on the cytokine regulation of intestinal immune responses to Eimeria. Poultry Sci 83:611-23View Article
  Luiz WB, Cavalcante RC, Paccez JD, Souza RD, Sbrogio-Almeida ME, Ferreira RC, Ferreira LC (2008) Boosting systemic and secreted antibody responses in mice orally immunized with r
• 作者单位：Zhiwei Lin (1)
  Yanyun Shi (1)
  Bin Deng (1)
  Xiangfei Mao (1)
  Dongyou Yu (1)
  Weifen Li (1)
  
  1. Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, 388 Yuhangtang Road, West Lake District, Hangzhou, 310058, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

The current experiment was conducted to construct recombinant Bacillus subtilis WB600 expressing Eimeria tenella 3-1E protein to investigate the oral immunization protective effects against E. tenella. The merozoite surface antigen 3-1E gene of E. tenella was introduced into the pBS-H1 expression vector with a novel signal peptide sequence. After the electro-transformation, the expression of objective protein in B. subtilis WB600 was detected by Western blot. The results showed that the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Seven-day-old broiler chickens were orally vaccinated with B. subtilis WB600 harboring 3-1E (B.S-pBS-H1-3-1E) or B. subtilis WB600 with empty plasmid (B.S-pBS-H1) 10?days prior to challenge with sporulated E. tenella oocysts. The results showed the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Vaccination with B.S-pBS-H1-3-1E strain significantly increased the anti-coccidial index and reduced cecal lesion scores compared with the positive control group (chickens were challenged with sporulated E. tenella oocysts without oral administration of B.S-pBS-H1-3-1E strain) and B.S-pBS-H1 group. Ceca mucosal sIgA, secretion, and IL-2, IL-12, IFN-γ, and IL-10 level after challenge were greater in the B.S-pBS-H1-3-1E group than in the positive control group. Taken together, these results indicated that B. subtilis WB600 harboring 3-1E protein induces protective immunity against E. tenella.