Eimeria tenella: a novel dsRNA virus in E. tenella and its complete genome sequence analysis

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• 作者：Bin Wu ; Xichen Zhang ; Pengtao Gong ; Mingying Li ; He Ding ; Caiyan Xin…
• 关键词：Eimeria tenella ; E. tenella RNA virus 1 ; Complete genome sequence ; Totiviridae ; Victorivirus ; Eimeriaviruses
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：52
• 期：2
• 页码：244-252
• 全文大小：1,546 KB
• 参考文献：1.ICTV 2014 Master Species List (MSL)
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• 作者单位：Bin Wu (1)
  Xichen Zhang (1)
  Pengtao Gong (1)
  Mingying Li (1)
  He Ding (1)
  Caiyan Xin (1)
  Na Zhao (1)
  Jianhua Li (1)
  
  1. College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X

文摘

Protozoa double-stranded (ds) RNA viruses have been described in Trichomonas, Giardia, and Leishmania. In this study, dsRNA and virus-like particles (approximately 30 nm in diameter) were discovered in Eimeria tenella sporulated oocysts. The complete genome of this novel dsRNA virus was sequenced using a three-step strategy. The sequencing results showed that the complete genome sequence was 6006 bp containing two open reading frames (ORFs) (2367 bp for ORF1 and 3216 bp for ORF2) with a five-nucleotide overlap (UGA/UG). The predicted ORF1 and ORF2 encoded a putative capsid protein of 788 amino acids (84.922 kDa) and a putative RNA-dependent RNA polymerase (RdRp) protein of 1071 amino acids (118.190 kDa). BLASTp analysis showed that the amino acid sequences for the E. tenella virus shared similarity with the E. brunetti RNA virus, with 29% homology in capsid proteins and 36% in RDRP proteins. The two untranslated regions were 349 bp (5′ UTR) and 78 bp (3′ UTR). The complete genome sequence of the E. tenella virus resembled characteristics of the Totiviridae family, indicating that this virus was a novel member of Totiviridae. Surprisingly, phylogenetic analysis showed that the E. tenella virus, E. brunetti RNA virus 1, and Mycoviruses were clustered into the genus Victorivirus and separated from the reported protozoa viruses, strongly suggesting a novel Eimeriaviruses subgenus. To the best of our knowledge, this is the first report for the complete genome sequence of the E. tenella virus. Using the nomenclature generally adopted for viruses, this new isolate was named E. tenella RNA virus 1. This study provides a foundation basis for further research on the biological characteristics of Eimeriaviruses.